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HRP-polymer secondary antibodies use micropolymer technology to form smaller detection complexes that allow better tissue penetration and sensitivity. In addition, HRP-polymer secondaries bind more horseradish peroxidase than standard HRP secondary antibodies, increasing signal.
Additional steps for biotin blocking may be required
On average 1 hour less
Simplified protocol means quicker results with HRP-polymer secondaries.
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Higher number of HRP molecules bound to HRP-polymer antibodies increase their sensitivity
Potential background from endogenous biotin
No background from endogenous biotin
Using HRP-polymer secondary antibodies eliminates biotin background.
Great for tissues with high biotin.
Example of IHC-parafin using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879
Nuclear detection of Ki67 by immunohistochemistry on breast cancer paraffin embedded tissue using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879