For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

Secondary antibodies optimized for co-immunoprecipitation western blotting

Related

  • Secondary antibodies
    • Alexa Fluor® secondaries
      • HRP conjugated
        • Biotinylated secondaries
          • Antibodies optimized for co-IP western blotting
            • Cell Imaging guide
              • Fluorochrome chart
                • Compare Alexa Fluor®488
                  • Advantages of Alexa Fluor® secondaries
                      • IP products
                        • IP protocol
                          • IP troubleshooting
                            • Veriblot IP secondaries
                              • Using IgM antibodies for IP
                                  • Western blot tools
                                    • Infrared fluorescence vs chemiluminescence

                                        ​

                                        Identify and isolate physiologically relevant proteins bound to your target protein using co-immunoprecipitation (co-IP), and uncover protein-protein interactions without interference from primary antibody heavy and light chain after co-IP steps..


                                        Common problems associated with secondary antibodies for western blotting, after the co-IP step, are the signals generated as a result of the denatured primary antibody heavy (~50 kDa) or light chains (~25 kDa). This can cause a masking effect, making it difficult to detect your protein of interest if it migrates to around either the 50 kDa or 25 kDa markers.

                                        To overcome this masking issue, we have developed secondary antibodies optimized for co-IP that only recognize primary antibodies in their native state. This eliminates the detection of denatured primary heavy and/or light chains during western blotting. 

                                        Furthermore, these optimized secondary antibodies are conjugated to horseradish peroxidase (HRP), alkaline phosphatase (AP), or biotin – all ideal for western blotting.

                                        When choosing secondary antibodies optimized for co-IP, it is important to take into consideration the size of your protein of interest.
                                        ​

                                        Secondary antibodies optimized for co-IP for full-length intact proteins of any size

                                        Product nameConjugateProduct code
                                        Anti-goat IgG VeriBlot for IP secondary antibodyHRPab157532
                                        Anti-mouse IgG VeriBlot for IP secondary antibodyBiotinab131367
                                        Anti-mouse IgG VeriBlot for IP secondary antibodyHRPab131368
                                        IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheepAPab131365
                                        IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheepHRPab131366


                                        ​Light chain-specific secondary antibodies for proteins of interest, greater than 30 kDa

                                        Product nameConjugateProduct code
                                        Rat anti-mouse kappa light chainAPab99616
                                        Rat anti-mouse lambda light chainAPab99624
                                        Rat anti-mouse kappa light chainAPab99631
                                        Mouse anti-rabbit IgG light chainAPab99696
                                        Mouse anti-rat kappa light chainAPab99691
                                        Mouse anti-human lambda light chainHRPab99811
                                        Rat anti-mouse kappa light chainHRPab99617
                                        Rat anti-mouse lambda light chainHRPab99632
                                        Mouse anti-rabbit IgG light chainHRPab99697
                                        Mouse anti-rat kappa light chainHRPab99692

                                        Browse over 2,500 high-quality secondary antibodies.

                                        See next immunoprecipitation protocol
                                        Get resources and offers direct to your inbox Sign up
                                        A-Z by research area
                                        • Cancer
                                        • Cardiovascular
                                        • Cell biology
                                        • Developmental biology
                                        • Epigenetics & Nuclear signaling
                                        • Immunology
                                        • Metabolism
                                        • Microbiology
                                        • Neuroscience
                                        • Signal transduction
                                        • Stem cells
                                        A-Z by product type
                                        • Primary antibodies
                                        • Secondary antibodies
                                        • Biochemicals
                                        • Isotype controls
                                        • Flow cytometry multi-color selector
                                        • Kits
                                        • Loading controls
                                        • Lysates
                                        • Peptides
                                        • Proteins
                                        • Slides
                                        • Tags and cell markers
                                        • Tools & Reagents
                                        Help & support
                                        • Support
                                        • Make an Inquiry
                                        • Protocols & troubleshooting
                                        • Placing an order
                                        • RabMAb products
                                        • Biochemical product FAQs
                                        • Training
                                        • Browse by Target
                                        Company
                                        • Corporate site
                                        • Investor relations
                                        • Company news
                                        • Careers
                                        • About us
                                        • Blog
                                        Events
                                        • Tradeshows
                                        • Conferences
                                        International websites
                                        • abcam.cn
                                        • abcam.co.jp

                                        Join with us

                                        • LinkedIn
                                        • facebook
                                        • Twitter
                                        • YouTube
                                        • Terms of sale
                                        • Website terms of use
                                        • Cookie policy
                                        • Privacy policy
                                        • Legal
                                        • Modern slavery statement
                                        © 1998-2021 Abcam plc. All rights reserved.