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Secondary antibodies optimized for co-immunoprecipitation western blotting

Related

  • Secondary antibodies
    • Alexa Fluor® secondaries
      • HRP conjugated
        • Biotinylated secondaries
          • Antibodies optimized for co-IP western blotting
            • Cell Imaging guide
              • Fluorochrome chart
                • Compare Alexa Fluor®488
                  • Advantages of Alexa Fluor® secondaries
                      • Immunoprecipitation (IP)
                        • Webinar: Introduction to IP
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                                • Western blot tools
                                  • Infrared fluorescence vs chemiluminescence
                                    • InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

                                      ​Identify and isolate physiologically relevant proteins bound to your target protein using co-immunoprecipitation (co-IP), and uncover protein-protein interactions without interference from primary antibody heavy and light chain after co-IP steps.

                                      Common problems associated with secondary antibodies for western blotting, after the co-IP step, are the signals generated as a result of the denatured primary antibody heavy (~50 kDa) or light chains (~25 kDa). This can cause a masking effect, making it difficult to detect your protein of interest if it migrates to around either the 50 kDa or 25 kDa markers.

                                      To overcome this masking issue, we have developed secondary antibodies optimized for co-IP that only recognize primary antibodies in their native state. This eliminates the detection of denatured primary heavy and/or light chains during western blotting. 

                                      Furthermore, these optimized secondary antibodies are conjugated to horseradish peroxidase (HRP), alkaline phosphatase (AP), or biotin – all ideal for western blotting.

                                      When choosing secondary antibodies optimized for co-IP, it is important to take into consideration the size of your protein of interest.
                                      ​

                                      Secondary antibodies optimized for co-IP for full-length intact proteins of any size

                                      Product nameConjugateProduct code
                                      Anti-goat IgG VeriBlot for IP secondary antibodyHRPab157532
                                      Anti-mouse IgG VeriBlot for IP secondary antibodyBiotinab131367
                                      Anti-mouse IgG VeriBlot for IP secondary antibodyHRPab131368
                                      IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheepHRPab131366


                                      ​Light chain-specific secondary antibodies for proteins of interest, greater than 30 kDa

                                      Product nameConjugateProduct code
                                      Rat anti-mouse lambda light chainAPab99624
                                      Mouse anti-rabbit IgG light chainAPab99696
                                      Mouse anti-human lambda light chainHRPab99811
                                      Mouse anti-rabbit IgG light chainHRPab99697

                                      Browse over 2,500 high-quality secondary antibodies.

                                      See next immunoprecipitation protocol
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