Secondary antibodies optimized for co-immunoprecipitation western blotting
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Identify and isolate physiologically relevant proteins bound to your target protein using co-immunoprecipitation (co-IP), and uncover protein-protein interactions without interference from primary antibody heavy and light chain after co-IP steps.
Common problems associated with secondary antibodies for western blotting, after the co-IP step, are the signals generated as a result of the denatured primary antibody heavy (~50 kDa) or light chains (~25 kDa). This can cause a masking effect, making it difficult to detect your protein of interest if it migrates to around either the 50 kDa or 25 kDa markers.
To overcome this masking issue, we have developed secondary antibodies optimized for co-IP that only recognize primary antibodies in their native state. This eliminates the detection of denatured primary heavy and/or light chains during western blotting.
Furthermore, these optimized secondary antibodies are conjugated to horseradish peroxidase (HRP), alkaline phosphatase (AP), or biotin – all ideal for western blotting.
When choosing secondary antibodies optimized for co-IP, it is important to take into consideration the size of your protein of interest.
Secondary antibodies optimized for co-IP for full-length intact proteins of any size
| Product name | Conjugate | Product code |
| Anti-goat IgG VeriBlot for IP secondary antibody | HRP | ab157532 |
| Anti-mouse IgG VeriBlot for IP secondary antibody | Biotin | ab131367 |
| Anti-mouse IgG VeriBlot for IP secondary antibody | HRP | ab131368 |
| IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheep | HRP | ab131366 |
Light chain-specific secondary antibodies for proteins of interest, greater than 30 kDa
| Product name | Conjugate | Product code |
| Rat anti-mouse lambda light chain | AP | ab99624 |
| Mouse anti-rabbit IgG light chain | AP | ab99696 |
| Mouse anti-human lambda light chain | HRP | ab99811 |
| Mouse anti-rabbit IgG light chain | HRP | ab99697 |