Secondary antibodies tested with Abcam primary antibodies
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Recombinant primary antibodies validated in immunofluorescence are tested with our Alexa Fluor® secondary antibodies. To make sure you get the faster results in immunostaining with your primary antibody, get the tested secondary antibody too.
See Alexa Fluor dyes such as Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 emission spectrum, compatible Alexa Fluor secondary antibodies and customer reviews on secondary antibodies for primary antibodies raise in rabbit and mouse. See by using tested Alexa Fluor® secondary antibodies for immunofluorescence with recombinant primary antibodies the other customers got bright cell or tissue fluorescent staining.
Alexa Fluor® 488 low self-quenching, high fluorescence quantum yield allow bright and sensitive cell staining. Absorption at 495 nm and emission maximum at 519 nm spectrum. For fluorescent cell and tissue staining of multiple proteins use in conjugation with secondaries conjugated to Alexa Fluor® 594 or Alexa Fluor® 647 conjugated secondary antibodies
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Customer testimonial (5 stars) - 'Excellent! Bright, clear staining observed with secondary antibody ab150077 at a 1:200 dilution.'
Alexa Fluor® 594
Alexa Fluor® 594 spectrum, absorption at 561 nm and emission maximum at 594 nm spectrum make it ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor® 488 and Alexa Fluor® 647 conjugated secondary antibodies
Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
Customer testimonial (5 stars) - 'Very good antibody
1ST AB used: Rat anti-ApoE'
Alexa Fluor® 647
Alexa Fluor® 647 spectrum, absorption at 650 nm and emission maximum at 665 nm spectrum make it ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor® 488 and Alexa Fluor® 594 conjugated secondary antibodies
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
Customer testimonial (5 stars) - 'My go-to secondary for IHC. I use a 1:500 dilution in 1XPBS.'
Other recommended secondary antibodies
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Customer testimonial (5 stars) - 'Worked good for DAB staining in placenta for neutrophils 1:200 dilution for 1 hour'
See all secondary antibodies
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
