Anti-Secretogranin V antibody (ab22699)
Key features and details
- Rabbit polyclonal to Secretogranin V
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Secretogranin V antibody
See all Secretogranin V primary antibodies -
Description
Rabbit polyclonal to Secretogranin V -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide:
EQLGIARPRV EYPAHQA
conjugated to BSA by a CDI linker, corresponding to N terminal amino acids 23-39 of Pig Secretogranin V (45-61 of pro-Pig Secretogranin V). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading... -
Purification notes
Partially purified, caprylic acid and ammonium sulphate precipitative clean-up. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. -
Sequence similarities
Belongs to the 7B2 family. -
Post-translational
modificationsProteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.
Sulfated on tyrosine residues. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 6447 Human
- Entrez Gene: 19064 Mouse
- Entrez Gene: 20394 Mouse
- Entrez Gene: 24677 Rat
- Entrez Gene: 25719 Rat
- Omim: 173120 Human
- SwissProt: P05408 Human
- SwissProt: P10601 Mouse
see all -
Alternative names
- 7B2 antibody
- 7B2 protein antibody
- 7B2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretogranin V antibody (ab22699)ab75197 (1µg/ml) staining secretogranin V in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of Islets of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Secretogranin V antibody (ab22699)ICC/IF image of ab22699 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab22699 has been referenced in 3 publications.
- Zheng B et al. A three-gene panel that distinguishes benign from malignant thyroid nodules. Int J Cancer 136:1646-54 (2015). PubMed: 25175491
- Sato A et al. Distribution of 7B2 (secretogranin V)-like immunoreactivity in the Japanese red-bellied newt (Cynops pyrrhogaster) pituitary. Tissue Cell 42:176-80 (2010). IHC-Fr ; Newt . PubMed: 20434189
- McGrath MF & de Bold AJ Transcriptional analysis of the mammalian heart with special reference to its endocrine function. BMC Genomics 10:254 (2009). IHC . PubMed: 19486520
