Overview

  • Product name

    Anti-Secretory phospholipase A2 antibody
    See all Secretory phospholipase A2 primary antibodies
  • Description

    Goat polyclonal to Secretory phospholipase A2
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Secretory phospholipase A2 aa 85-96 (internal sequence) (Cysteine residue).
    Sequence:

    SYKFSNSGSRIT


    Database link: P14555

  • Positive control

    • Human placenta lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab205399 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 16 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.1.1.4 antibody
    • GIIC sPLA2 antibody
    • Group IIA phospholipase A2 antibody
    • membrane associated antibody
    • Modifier of MIN 1 mouse homolog antibody
    • MOM1 antibody
    • Non-pancreatic secretory phospholipase A2 antibody
    • NPS PLA2 antibody
    • NPS-PLA2 antibody
    • PA2GA_HUMAN antibody
    • Phosphatidylcholine 2-acylhydrolase 2A antibody
    • Phosphatidylcholine 2acylhydrolase antibody
    • Phospholipase A2 antibody
    • Phospholipase A2 group IIA (platelets, synovial fluid) antibody
    • Phospholipase A2 membrane associated antibody
    • Phospholipase A2 polypeptide B antibody
    • Phospholipase A2 synovial antibody
    • PLA2 antibody
    • PLA2B antibody
    • PLA2G2A antibody
    • PLA2L antibody
    • PLA2S antibody
    • PLAS1 antibody
    • RASF A antibody
    • sPLA2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine staining Secretory phospholipase A2 with ab205399 at 5 μg/ml

  • Anti-Secretory phospholipase A2 antibody (ab205399) at 0.1 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 16 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human liver staining Secretory phospholipase A2 with ab205399 at 5 μg/ml

References

ab205399 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205399.
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