Overview

  • Product name

    Anti-Secretory phospholipase A2 antibody
    See all Secretory phospholipase A2 primary antibodies
  • Description

    Rabbit polyclonal to Secretory phospholipase A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Does not react with: Rabbit, Cow, Pig
  • Immunogen

    Human recombinant sPLA2 (Type IIa).

Applications

Our Abpromise guarantee covers the use of ab23705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 14 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.1.1.4 antibody
    • GIIC sPLA2 antibody
    • Group IIA phospholipase A2 antibody
    • membrane associated antibody
    • Modifier of MIN 1 mouse homolog antibody
    • MOM1 antibody
    • Non-pancreatic secretory phospholipase A2 antibody
    • NPS PLA2 antibody
    • NPS-PLA2 antibody
    • PA2GA_HUMAN antibody
    • Phosphatidylcholine 2-acylhydrolase 2A antibody
    • Phosphatidylcholine 2acylhydrolase antibody
    • Phospholipase A2 antibody
    • Phospholipase A2 group IIA (platelets, synovial fluid) antibody
    • Phospholipase A2 membrane associated antibody
    • Phospholipase A2 polypeptide B antibody
    • Phospholipase A2 synovial antibody
    • PLA2 antibody
    • PLA2B antibody
    • PLA2G2A antibody
    • PLA2L antibody
    • PLA2S antibody
    • PLAS1 antibody
    • RASF A antibody
    • sPLA2 antibody
    see all

Images

  • Western blot detection of human secretory phospholipase A2 (25µg)

    ab23705 at 1/1000 western blot detection of 0.25 ug of human secretory phospolipase A2.
  • IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Valkonen S  et al. Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules. Biochim Biophys Acta Mol Cell Biol Lipids 1864:1168-1182 (2019). Read more (PubMed: 30980920) »
  • Kuefner MS  et al. Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism. J Lipid Res 58:1822-1833 (2017). Read more (PubMed: 28663239) »
See all 7 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Gingiva)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Gingiva
Blocking step
Generic block reagent as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 25 2017

Answer

Vielen Dank für Ihren Anruf.

Das Labor, das Anti-Phospholipase A2Antikoerper ab23705 herstellt, hat bestätigt, dass das Immunogen das Volllängenprotein sPLA2 (human Type IIA) mit der SwissProtID P14555.2 ist. Damit ist dieser Antikörper spezifisch für die Membran-assoziierte PLA2.

Die Antikörper ab124230 und ab73406 sind hingegen spezifisch für die cytosolische PLA2 mit der SwissProtID P47712.

Da diese beiden Formen sehr unterschiedlich sind, haben wir leider keinen Antikörper verfügbar, der beide Formen erkennt.

Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for contacting Abcam.

Under our Abpromise we guarantee our products to work as stated on the datasheet and if they do not work as stated, and we cannot resolve the issue you are having with the antibody then we would be happy to either send a replacement antibody or to process a refund.

In the case of ab23705, we guarantee to work in IHC-P, WB, IP on rat and human tissue. On the webpage for this product we have a Abreview and a publication that have used the antibody successfully in IHC-P in pancreatic tissue. However we have not tested it on brain sections.

Please let me know if there is anything else I can help you with.

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Question

Hi. I am inquiring about the sPLA2 rabbit poly-clonal antibody and its use in IHC-P. I have attempted numerous studies on human brain tissues using sPLA2 antibodies from other vendors and have not had consistent results and am considering trying AbCam. There is one published article from China in which they uses the antibody on rats pancreas but did not give details on which antigen retrieval buffer they used nor the source of their secondary antibody. Additionally, they incubated at 37C for all three steps. Your data information sheet is from formalin-fixed human tissue, using citrate buffer for AR, a short primary antibody incubation step at room temperature, but the secondary/tertiary reagents are not typical of what I have available. I will be using a manual LSAB system (we are a laboratory with somewhat limited funds at the moment), Tris/Tween buffer wash (I noticed that your standard protocol calls for Triton X) with DAB as the chromagen. I have read your information on IHC protocols but not sure what the data sheet for ab23705 is referring to for "protocol F". Do you think I will have success with your antibody or will I need to move up to a more sensitive detection system? There is not a lot of published results for sPLA2 IHC on human formalin-fixed tissues for me to ascertain whether my normal IHC protocol will be successful. I usually go overnight with the primary antibody at either 4C or room temperature. I would appreciate additional information on the HRP conjugated compact polymer system that you utilized in your studies for development of the antibody. Thanks much for any suggestions that you may have as I try to convince my PIs that we need to consider a new source for the sPLA2 antibody.

Read More
Answer

Thank you for contacting us. This product should work well with your conditions but of course must be optimized by the customer. This product was tested with short incubation time but may be used overnight at 4 degrees if you find you need a stronger signal. We do guarantee our products to work in our tested spcies and applications and will be happy to troubleshoot any product you are not having success with. We also can provide a free of charge replacement, credit, or refund within 6 months of purchase if the product is not working well. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your phone call today and letting us know about the problem with ab23705. Your credit note ID is ***. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further assistance.

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Answer

Thank you for your enquiry. Yes, this antibody does appear to be suitable for your needs. Ab23705 has been tested in Western blotting with rat samples and the antibody recognizes type IIa. If you have any additional questions, please contact us again.

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