Overview

  • Product name

    Secretory Phospholipase A2 Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Plasma
  • Assay type

    Enzyme activity (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Secretory Phospholipase A2 Assay Kit (ab133089) provides an accurate and convenient method for measurement of secretory PLA2 (sPLA2) activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5’-dithio-bis-(2-nitrobenzoic acid)).

    If you want to detect cytosolic PLA2, we recommend using Cytosolic Phospholipase A2 Assay Kit (ab133090).

  • Platform

    Microplate reader

Properties

Images

  • Absorbance times for bee venom, snake venom, human synovial, and bovine pancreas PLA2s.

Protocols

References

This product has been referenced in:

  • Tamayose CI  et al. Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28906474) »
  • Lewin M  et al. Varespladib (LY315920) Appears to Be a Potent, Broad-Spectrum, Inhibitor of Snake Venom Phospholipase A2 and a Possible Pre-Referral Treatment for Envenomation. Toxins (Basel) 8:N/A (2016). Read more (PubMed: 27571102) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I am sorry to confirm that we do not offer a kit for the measurement of the activity specifically of Lp-PLA2 (phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) = Lipoprotein-associated phospholipase A2 = PLA2G7).

Cytosolic Phospholipase A2 Assay Kit (ab133090) can be used to determine the activity of cytosolic Phospholipase A2 (cPLA2, PLA2G4 = Phospholipase A2, group IVA (cytosolic, calcium-dependent)) in purified preparations, cell cultures, or tissue homogenates.
Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone.

Maybe this kit would be suitable for you?

Please review the datasheet to confirm that this kit would be suitable for your experiments.

https://www.abcam.com/index.html?datasheet=133090 (or use the following: https://www.abcam.com/index.html?datasheet=133090).

I am happy to confirm that we sell directly to Greece. Please set your location on our homepage to Greece (in the right top corner). This will provide country specific information about prices and shipping, etc.

I hope this information is helpful and wish you good luck with your experiments.

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