Anti-Secretory phospholipase A2 Type V antibody (ab23709)


  • Product name
    Anti-Secretory phospholipase A2 Type V antibody
    See all Secretory phospholipase A2 Type V primary antibodies
  • Description
    Rabbit polyclonal to Secretory phospholipase A2 Type V
  • Host species
  • Specificity
    (+) Human, murine, and rat sPLA2 (Type V); (-) bee venom and human synovial sPLA2 (Type II), iPLA2 (A10 cell lysate), and cPLA2 (HeLa cell lysate); sPLA2 (Type X) not tested, but is expected to be negative (peptide antigen is 12.5% homologous to murine and rat sPLA2 (Type X) and 25% homologous to human (Type II) and (Type X) sPLA2).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 79-94 of Mouse Secretory phospholipase A2 Type V.

  • Positive control
    • Expressed in heart, lung, placenta and spleen. IHC-P: FFPE human heart tissue sections.


Our Abpromise guarantee covers the use of ab23709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/500. Predicted molecular weight: 15 kDa (Human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
    • Tissue specificity
      Heart, placenta and less abundantly, in lung.
    • Sequence similarities
      Belongs to the phospholipase A2 family.
    • Post-translational
      This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Ca2+ dependent phospholipase A2 antibody
      • Calcium dependent phospholipase A2 antibody
      • Calcium-dependent phospholipase A2 antibody
      • DKFZp686C2294 antibody
      • FRFB antibody
      • Group V phospholipase A2 antibody
      • GV PLA2 antibody
      • gVPLA2 antibody
      • hVPLA(2) antibody
      • MGC46205 antibody
      • OTTHUMP00000044655 antibody
      • PA2G5_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase antibody
      • Phosphatidylcholine 2-acylhydrolase 5 antibody
      • Phospholipase A2 group V antibody
      • PLA2 10 antibody
      • PLA2 G5 antibody
      • PLA2-10 antibody
      • Pla2g5 antibody
      • sPLA2 Type V antibody
      see all


    • IHC image of Secretory phospholipase A2 Type V staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Secretory phospholipase A2 Type V antibody (ab23709) at 1/500 dilution

      Lane 1 : Recombinant human sPLA2 (type V) (100 ng)
      Lane 2 : Recombinant human sPLA2 (type V) (50 ng)
      Lane 3 : Mouse heart homogenate (50ug)

      Predicted band size: 15 kDa
      Observed band size: 15 kDa


    This product has been referenced in:
    • Zhu S  et al. High-Density Lipoprotein (HDL) Counter-Regulates Serum Amyloid A (SAA)-Induced sPLA2-IIE and sPLA2-V Expression in Macrophages. PLoS One 11:e0167468 (2016). WB ; Mouse . Read more (PubMed: 27898742) »

    See 1 Publication for this product

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