Overview

  • Product name

    Anti-Securin 2 antibody
  • Description

    Rabbit polyclonal to Securin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 2 and 174 of Human Securin 2.

  • Positive control

    • NT2D1 whole cell lysate, IMR32 and U-87 MG cell lysates; Human breast carcinoma tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed at low levels in the pituitary, liver, spleen, prostate, testis, ovary, small intestine and colon. Also expressed in various pituitary, testicular, liver and ovarian tumors.
  • Sequence similarities

    Belongs to the securin family.
  • Domain

    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Pituitary tumor transforming 2 antibody
    • Pituitary tumor transforming gene 2 protein antibody
    • Pituitary tumor-transforming gene 2 protein antibody
    • PTTG2 antibody
    • PTTG2_HUMAN antibody
    • Securin-2 antibody
    see all

Images

  • Anti-Securin 2 antibody (ab127039) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE
  • ab127039, at a 1/250 dilution, staining Securin 2 in paraffin embedded Human breast canrcinoma tissue by Immunohistochemistry.

References

ab127039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The antibody ab127039 was generated by an immunogen derived from human securin 2. However, the immunogen's sequence of amino acids shares 91% identity with securin 1. We do not have data demonstrating relative affinity of the antibody for 1 and 2 but reaction with both seems likely, based on the similarity of the sequences.


Also, the western blot data from NT2D1 whole cell lysate on the datasheet shows detection of two bands, which might be 1 and 2. The Uniprot records for the two proteins show that the molecular weights predicted based on the amino acid sequences of the precursors differ by about 0.3 kDa.


http://www.uniprot.org/uniprot/Q9NZH5#sequences


http://www.uniprot.org/uniprot/O95997#sequences

Read More

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