Overview

  • Product name

  • Description

    Rabbit polyclonal to Securin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Securin aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: O95997

  • Positive control

    • IHC-P: Human rectal carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • Tissue specificity

    Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • Sequence similarities

    Belongs to the securin family.
  • Developmental stage

    Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • Domain

    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • Post-translational
    modifications

    Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW555095 antibody
    • C87862 antibody
    • Cut2 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ESP1 associated protein 1 antibody
    • Esp1-associated protein antibody
    • hPTTG antibody
    • MGC126883 antibody
    • MGC138276 antibody
    • Pds1 antibody
    • Pituitary tumor transforming 1 antibody
    • Pituitary tumor transforming protein 1 antibody
    • Pituitary tumor-transforming 1, isoform CRA_a antibody
    • Pituitary tumor-transforming 1, isoform CRA_b antibody
    • Pituitary tumor-transforming gene 1 antibody
    • Pituitary tumor-transforming gene 1 protein antibody
    • PTTG 1 antibody
    • PTTG antibody
    • PTTG1 antibody
    • PTTG1 protein antibody
    • PTTG1_HUMAN antibody
    • Pttg3 antibody
    • Securin antibody
    • Tumor transforming 1 antibody
    • Tumor transforming protein 1 antibody
    • Tumor-transforming protein 1 antibody
    • TUTR 1 antibody
    • TUTR1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human rectal carcinoma tissue stained for Securin using ab227773 at 1/100 dilution in immunohistochemical analysis.

References

ab227773 has not yet been referenced specifically in any publications.

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