Product nameAnti-Securin antibody
See all Securin primary antibodies
DescriptionRabbit polyclonal to Securin
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Securin.
- Recombinant Human Securin protein (ab116478) can be used as a positive control in WB. This antibody gave a positive signal in HEK293 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab26273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).|
FunctionRegulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
Tissue specificityExpressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
Sequence similaritiesBelongs to the securin family.
Developmental stageLow level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
DomainThe N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
modificationsPhosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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HeLa cells stained with ab26273 (1/100 dilution). A clear nuclear signal in interphase HeLa cells can be seen.
All lanes : Anti-Securin antibody (ab26273) at 1/500 dilution
Lane 1 : HEK293 human cell lysate
Lane 2 : MPRO mouse cell lysate
Lane 3 : S. Cerevisiae lysate
Lysates/proteins at 50 µg per lane.
All lanes : Polyclonal Goat anti Rabbit at 1/10000 dilution
Predicted band size: 22 kDa
Anti-Securin antibody (ab26273) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
ab26273 detects a clean 26 kDa band in HEK293 lysate. We would recommend loading 20-40ug of protein when using this antibody. ab26273 has also been tested in mouse lysates and in our hands detects a 70 kDa band which we cannot explain.
This product has been referenced in:
- Lee SB et al. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1. Mol Cell 60:21-34 (2015). IP . Read more (PubMed: 26387737) »
- Lubka-Pathak M et al. Altered expression of securin (Pttg1) and serpina3n in the auditory system of hearing-impaired Tff3-deficient mice. Cell Mol Life Sci 68:2739-49 (2011). Read more (PubMed: 21076990) »