Recombinant
RabMAb

Recombinant Anti-SEH1L antibody [EPR20851] - BSA and Azide free (ab235840)

Overview

  • Product name

    Anti-SEH1L antibody [EPR20851] - BSA and Azide free
    See all SEH1L primary antibodies
  • Description

    Rabbit monoclonal [EPR20851] to SEH1L - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Mouse SEH1L aa 1-100. The exact sequence is proprietary.
    Database link: Q8R2U0

  • Positive control

    • WB: HEK-293T whole cell lysate.
  • General notes

    Ab235840 is the carrier-free version of ab218531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235840 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore.
  • Sequence similarities

    Belongs to the WD repeat SEC13 family.
    Contains 6 WD repeats.
  • Cellular localization

    Chromosome > centromere > kinetochore. Nucleus > nuclear pore complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • Nucleoporin SEH1 antibody
    • Nup107-160 subcomplex subunit SEH1 antibody
    • SEC13-like protein antibody
    • SEC13L antibody
    • Seh 1 antibody
    • SEH1-like (S. cerevisiae) antibody
    • SEH1_HUMAN antibody
    • SEH1A antibody
    • SEH1B antibody
    • SEH1L antibody
    see all

Images

  • SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HEK-293T whole cell lysate 10 µg (Input).
    Lane 2: ab218531 IP in HEK-293T whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315).

    According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

  • All lanes : Anti-SEH1L antibody [EPR20851] (ab218531) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.

    The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

     

References

ab235840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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