Overview

  • Product name

  • Description

    Rabbit polyclonal to Seladin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55-358 of Human Seladin 1 (UniProt ID: Q15392).

  • Positive control

    • A431, H1299 and mouse brain lysates; HeLa cell; SW480 xenograft

Applications

Our Abpromise guarantee covers the use of ab137845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.

Target

  • Function

    Catalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.
  • Tissue specificity

    Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.
  • Pathway

    Steroid biosynthesis; cholesterol biosynthesis.
  • Involvement in disease

    Desmosterolosis (DESMOS) [MIM:602398]: Rare autosomal recessive disorder characterized by multiple congenital anomalies and elevated levels of the cholesterol precursor desmosterol in plasma, tissue, and cultured cells. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
    Contains 1 FAD-binding PCMH-type domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 24 dehydrocholesterol reductase antibody
    • 24-dehydrocholesterol reductase antibody
    • 3 beta hydroxysterol delta 24 reductase antibody
    • 3-beta-hydroxysterol delta-24-reductase antibody
    • DCE antibody
    • Delta(24)-sterol reductase antibody
    • Desmosterol to cholesterol enzyme antibody
    • DHC24_HUMAN antibody
    • Dhcr24 antibody
    • Diminuto dwarf1 homolog antibody
    • Diminuto/dwarf1 homolog antibody
    • KIAA0018 antibody
    • Nbla03646 antibody
    • Seladin-1 antibody
    • SELADIN1 antibody
    • Selective AD indicator 1 antibody
    • Selective Alzheimer's Disease Indicator 1 antibody
    see all

Images

  • Anti-Seladin 1 antibody (ab137845) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Anti-Seladin 1 antibody (ab137845) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cell labelling Seladin 1 with ab137845 at a 1/200 dilution. Lower panel merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded SW480 xenograft labelling Seladin 1 with ab137845 at a 1/100 dilution.

References

This product has been referenced in:

  • Zhang P  et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med 23:1055-1062 (2017). Read more (PubMed: 28805822) »
See 1 Publication for this product

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