Anti-Selenium Binding Protein 1/SBP antibody (ab180782)
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Overview
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Product name
Anti-Selenium Binding Protein 1/SBP antibody
See all Selenium Binding Protein 1/SBP primary antibodies -
Description
Rabbit polyclonal to Selenium Binding Protein 1/SBP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human Selenium Binding Protein 1/SBP (C terminal).
Database link: Q13228 -
Positive control
- 721_B cell lysate
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General notes
Previously labelled as Selenium Binding Protein 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab180782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/500 - 1/2000. Predicted molecular weight: 52 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport. -
Tissue specificity
Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients. -
Sequence similarities
Belongs to the selenium-binding protein family. -
Post-translational
modificationsPhosphorylated.
The N-terminus is blocked. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Membrane. May associate with Golgi membrane. May associate with the membrane of autophagosomes. - Information by UniProt
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Database links
- Entrez Gene: 510154 Cow
- Entrez Gene: 8991 Human
- Entrez Gene: 20341 Mouse
- Entrez Gene: 140927 Rat
- Omim: 604188 Human
- SwissProt: Q2KJ32 Cow
- SwissProt: Q13228 Human
- SwissProt: P17563 Mouse
see all -
Alternative names
- 56 kDa selenium binding protein antibody
- 56 kDa selenium-binding protein antibody
- hSBP antibody
see all
Images
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Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab180782)Anti-Selenium Binding Protein 1/SBP antibody (ab180782) at 1/500 dilution + 721_B cell lysate
Predicted band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab180782)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling Selenium Binding Protein 1/SBP with ab180782 at 1/200. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab180782)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Selenium Binding Protein 1/SBP with ab180782 at 1/200. Magnification: 400x.
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Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab180782)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180782. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
References
ab180782 has not yet been referenced specifically in any publications.
