Product nameAnti-SELK antibody
See all SELK primary antibodies
DescriptionRabbit polyclonal to SELK
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- RT 4, U 251 MG, Human liver and Human tonsil lysates; Human salivary gland tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the selenoprotein K family.
- Information by UniProt
- HSPC030 antibody
- HSPC297 antibody
- Selenoprotein K antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121276 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-SELK antibody (ab121276) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human Salivary gland tissue by Immunohistochemistry.
ab121276 has not yet been referenced specifically in any publications.