Key features and details
- Rabbit polyclonal to SELK
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SELK antibody
See all SELK primary antibodies
DescriptionRabbit polyclonal to SELK
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- ICC/IF: U-251 MG cells. WB: HepG2 cell lysate. IHC: Human tonsil and salivary gland tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the selenoprotein K family.
- Information by UniProt
- HSPC030 antibody
- HSPC297 antibody
- Selenoprotein K antibody
Immunofluorescent staining of U-251 MG (Human brain glioma) cells labeling SELK with ab121276 at 0.25 ug/ml (green).
Anti-SELK antibody (ab121276) at 0.04 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Performed under reducing conditions.
Western blot analysis of HepG2 cells labeling SELK with ab121276 at 0.04 ug/ml.
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human liver tissue by Immunohistochemistry.
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human testis tissue by Immunohistochemistry.
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
ab121276 has not yet been referenced specifically in any publications.