Recombinant Anti-SelM antibody [EPR8279] (ab133681)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8279] to SelM
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SelM antibody [EPR8279]
See all SelM primary antibodies -
Description
Rabbit monoclonal [EPR8279] to SelM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SelM aa 100-200 (C terminal).
Database link: Q8WWX9 -
Positive control
- Human ovary cancer and stomach lysates; Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8279 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Selenoprotein M is widely expressed and expressed highly in the mammalian brain. It is localized to the perinuclear structures (Golgi/ER). A growing body of evidence relates selenium to cancer prevention, immune system function, male fertility, cardiovascular disorder, control of the aging and neurodiseases process. Selenoproteins are thought to be responsible for the majority of these biomedical effects of selenium. Approximately 17 selenoproteins have been identified until now. Although the function of many selenoproteins are unknown, some play important roles in antioxidant mechanisms. It has been also implicated in the regulation of signaling pathways through catalysis of thiol/disulfide exchange. The roles of Selenoprotein M have not been clearly identified until present time. -
Cellular localization
Cytoplasm; perinuclear region. Endoplasmic reticulum Probable. Golgi apparatus Probable. Note: Localized to perinuclear structures corresponding to Golgi and endoplasmic reticulum. -
Database links
- Entrez Gene: 140606 Human
- Omim: 610918 Human
- SwissProt: Q8WWX9 Human
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Alternative names
- Selenoprotein M antibody
- Selenoprotein SelM antibody
- SELM antibody
see all
Images
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All lanes : Anti-SelM antibody [EPR8279] (ab133681) at 1/1000 dilution
Lane 1 : Human ovary cancer lysate
Lane 2 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SelM with ab133681 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab133681 has been referenced in 6 publications.
- Zhang DG et al. Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco. Antioxidants (Basel) 10:N/A (2021). PubMed: 33805536
- Sonet J et al. Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines. Int J Mol Sci 22:N/A (2021). PubMed: 34298926
- Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . PubMed: 29660373
- Ekoue DN et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). PubMed: 29314169
- Legrain Y et al. Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts. J Biol Chem 289:6299-310 (2014). WB ; Human . PubMed: 24425862
- Hudson TS et al. Selenoproteins reduce susceptibility to DMBA-induced mammary carcinogenesis. Carcinogenesis 33:1225-30 (2012). PubMed: 22436612