Overview

  • Product name

    Anti-SelM antibody [EPR8279]
    See all SelM primary antibodies
  • Description

    Rabbit monoclonal [EPR8279] to SelM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SelM aa 100-200 (C terminal).
    Database link: Q8WWX9

  • Positive control

    • Human ovary cancer and stomach lysates; Human brain tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Selenoprotein M

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Selenoprotein M is widely expressed and expressed highly in the mammalian brain. It is localized to the perinuclear structures (Golgi/ER). A growing body of evidence relates selenium to cancer prevention, immune system function, male fertility, cardiovascular disorder, control of the aging and neurodiseases process. Selenoproteins are thought to be responsible for the majority of these biomedical effects of selenium. Approximately 17 selenoproteins have been identified until now. Although the function of many selenoproteins are unknown, some play important roles in antioxidant mechanisms. It has been also implicated in the regulation of signaling pathways through catalysis of thiol/disulfide exchange. The roles of Selenoprotein M have not been clearly identified until present time.
    • Cellular localization

      Cytoplasm; perinuclear region. Endoplasmic reticulum Probable. Golgi apparatus Probable. Note: Localized to perinuclear structures corresponding to Golgi and endoplasmic reticulum.
    • Database links

    • Alternative names

      • Selenoprotein M antibody
      • Selenoprotein SelM antibody
      • SELM antibody
      • SelM protein antibody
      • SEPM antibody
      see all

    Images

    • All lanes : Anti-SelM antibody [EPR8279] (ab133681) at 1/1000 dilution

      Lane 1 : Human ovary cancer lysate
      Lane 2 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 16 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SelM with ab133681 at 1/50 dilution.

    References

    This product has been referenced in:

    • Touat-Hamici Z  et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . Read more (PubMed: 29660373) »
    • Ekoue DN  et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). Read more (PubMed: 29314169) »
    See all 4 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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