Recombinant
RabMAb

Anti-SELO antibody [EPR11968] (ab172957)

Overview

  • Product name
    Anti-SELO antibody [EPR11968]
  • Description
    Rabbit monoclonal [EPR11968] to SELO
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SELO aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BVL4

  • Positive control
    • Fetal kidney, 293T, A549 and U87-MG lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11968
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-SELO antibody [EPR11968] (ab172957) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T lysate
      Lane 3 : A549 lysate
      Lane 4 : U87-MG lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa

    References

    ab172957 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (whole body)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    40 µg
    Specification
    whole body
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 23 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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