Recombinant
RabMAb

Anti-SELO antibody [EPR11968] (ab172957)

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Overview

  • Product name
    Anti-SELO antibody [EPR11968]
  • Description
    Rabbit monoclonal [EPR11968] to SELO
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SELO aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BVL4

  • Positive control
    • Fetal kidney, 293T, A549 and U87-MG lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11968
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-SELO antibody [EPR11968] (ab172957)
      Western blot - Anti-SELO antibody [EPR11968] (ab172957)
      All lanes : Anti-SELO antibody [EPR11968] (ab172957) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T lysate
      Lane 3 : A549 lysate
      Lane 4 : U87-MG lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa

    References

    ab172957 has not yet been referenced specifically in any publications.

    Publishing research using ab172957? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-SELO antibody [EPR11968]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (whole body)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    40 µg
    Specification
    whole body
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

    Verified customer

    Submitted Apr 23 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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