Key features and details
- Rabbit polyclonal to SELS
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SELS antibody
See all SELS primary antibodies
DescriptionRabbit polyclonal to SELS
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
- HeLa, Jurkat and 293T whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab190247 was affinity purified using an epitope specific to SELS immobilized on solid support.
Our Abpromise guarantee covers the use of ab190247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 21 kDa.|
RelevanceInvolved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.
Cellular localizationEndoplasmic reticulum; endoplasmic reticulum membrane; single pass membrane protein.
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All lanes : Anti-SELS antibody (ab190247) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate in NETN buffer
Lane 2 : HeLa whole cell lysate in RIPA buffer
Lane 3 : 293T whole cell lysate in NETN buffer
Lane 4 : Jurkat whole cell lysate in RIPA buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 3 minutes
Immunoprecipitation analysis of HeLa cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling SELS using ab190247 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated SELS, ab190247 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
ab190247 has not yet been referenced specifically in any publications.