Anti-SELS antibody (ab223721)
Key features and details
- Rabbit polyclonal to SELS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SELS antibody
See all SELS primary antibodies -
Description
Rabbit polyclonal to SELS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human SELS aa 50 to the C-terminus.
Database link: Q9BQE4 -
Positive control
- IHC-P: Human small intestine and pancreas tissue. WB: HEK-293 ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Relevance
Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination. -
Cellular localization
Endoplasmic reticulum; endoplasmic reticulum membrane; single pass membrane protein. -
Database links
- Entrez Gene: 512276 Cow
- Entrez Gene: 55829 Human
- Entrez Gene: 100151836 Pig
- Omim: 607918 Human
- SwissProt: Q2KI76 Cow
- SwissProt: Q9BQE4 Human
- SwissProt: F1SR90 Pig
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Alternative names
- AD-015 antibody
- ADO15 antibody
- MGC104346 antibody
see all
Images
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Paraffin embedded human pancreas tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.
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Paraffin embedded human small intestine tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SELS (green) using ab223721 (4 µg/ml) in ICC/IF.
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Anti-SELS antibody (ab223721) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223721 has not yet been referenced specifically in any publications.