Key features and details
- Rabbit polyclonal to SELS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SELS antibody
See all SELS primary antibodies
DescriptionRabbit polyclonal to SELS
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
- IHC-P: Human small intestine tissue. WB: HEK-293, NIH/3T3 and NBT-II cell lysates. ICC/IF: U-251 MG cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceInvolved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.
Cellular localizationEndoplasmic reticulum; endoplasmic reticulum membrane; single pass membrane protein.
- AD-015 antibody
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Paraffin embedded human small intestine tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SELS (green) using ab223721 (4 µg/ml) in ICC/IF.
Anti-SELS antibody (ab223721) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 21 kDa
All lanes : Anti-SELS antibody (ab223721) at 1/100 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 21 kDa
ab223721 has not yet been referenced specifically in any publications.