Anti-SEMA3B antibody (ab48197)
Key features and details
- Rabbit polyclonal to SEMA3B
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SEMA3B antibody
See all SEMA3B primary antibodies -
Description
Rabbit polyclonal to SEMA3B -
Host species
Rabbit -
Specificity
This antibody is specific for SEMA 3B. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Zebrafish -
Immunogen
Synthetic peptide corresponding to Human SEMA3B aa 100-200.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: Tris glycine, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 51, 83 kDa.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 51, 83 kDa. |
Target
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Function
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
Tissue specificity
Expressed abundantly but differentially in a variety of neural and nonneural tissues. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 20347 Mouse
- SwissProt: Q0VGP2 Mouse
- SwissProt: Q62177 Mouse
- Unigene: 4083 Mouse
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Alternative names
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
see all
Images
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Anti-SEMA3B antibody (ab48197) at 1/5000 dilution + mouse E16 cerebellum lysate
Developed using the ECL technique.
Predicted band size: 51, 83 kDa
Observed band size: 51,83 kDa why is the actual band size different from the predicted?
A band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein.
Datasheets and documents
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Datasheet download
References (11)
ab48197 has been referenced in 11 publications.
- Iwakura T et al. Prevalence of neural epidermal growth factor-like 1- and exostosin 1/exostosin 2-associated membranous nephropathy: a single-center retrospective study in Japan. Sci Rep 12:2967 (2022). PubMed: 35194125
- Du Y et al. Hippocampal semaphorin 3B improves depression-like behaviours in mice by upregulating synaptic plasticity and inhibiting neuronal apoptosis. J Neurochem 163:133-148 (2022). PubMed: 35892177
- Sethi S et al. Semaphorin 3B-associated membranous nephropathy is a distinct type of disease predominantly present in pediatric patients. Kidney Int 98:1253-1264 (2020). PubMed: 32534052
- Mohan V et al. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex Through Semaphorin 3B. J Neurosci 39:6233-6250 (2019). PubMed: 31182634
- Guo W et al. MiR-6872 host gene SEMA3B and its antisense lncRNA SEMA3B-AS1 function synergistically to suppress gastric cardia adenocarcinoma progression. Gastric Cancer N/A:N/A (2019). PubMed: 30656427