Anti-SEMA3B antibody (ab88489)
Key features and details
- Rabbit polyclonal to SEMA3B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEMA3B antibody
See all SEMA3B primary antibodies -
Description
Rabbit polyclonal to SEMA3B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human SEMA3B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101720) -
Positive control
- This antibody gave a positive signal in Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
Tissue specificity
Expressed abundantly but differentially in a variety of neural and nonneural tissues. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 7869 Human
- Entrez Gene: 20347 Mouse
- Omim: 601281 Human
- SwissProt: Q13214 Human
- SwissProt: Q0VGP2 Mouse
- SwissProt: Q62177 Mouse
- Unigene: 82222 Human
- Unigene: 4083 Mouse
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Alternative names
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
see all
Images
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Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThe band observed at 50 kDa represents the secreted form of SEMA3B.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab88489 has not yet been referenced specifically in any publications.