Product nameAnti-SEMA3B antibody
See all SEMA3B primary antibodies
DescriptionRabbit polyclonal to SEMA3B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human SEMA3B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human brain tissue lysate.
This product was previously labelled as Semaphorin 3B
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).|
FunctionInhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Tissue specificityExpressed abundantly but differentially in a variety of neural and nonneural tissues.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
Cellular localizationSecreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
- Information by UniProt
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 50 kDa represents the secreted form of SEMA3B.
ab88489 has not yet been referenced specifically in any publications.