Key features and details
- Rabbit polyclonal to SEMA3B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEMA3B antibody
See all SEMA3B primary antibodies
DescriptionRabbit polyclonal to SEMA3B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human SEMA3B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human brain tissue lysate.
This product was previously labelled as Semaphorin 3B
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).|
FunctionInhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Tissue specificityExpressed abundantly but differentially in a variety of neural and nonneural tissues.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
Cellular localizationSecreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
- Information by UniProt
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 50 kDa represents the secreted form of SEMA3B.
ab88489 has not yet been referenced specifically in any publications.