Overview

  • Product name

  • Description

    Rabbit polyclonal to SEMA3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SEMA3B aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101720)

  • Positive control

    • This antibody gave a positive signal in Human brain tissue lysate.
  • General notes

     This product was previously labelled as Semaphorin 3B

     

Properties

Applications

Our Abpromise guarantee covers the use of ab88489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Tissue specificity

    Expressed abundantly but differentially in a variety of neural and nonneural tissues.
  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localization

    Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • SEM3B_HUMAN antibody
    • Sema A(V) antibody
    • Sema V antibody
    • Sema3b antibody
    • Semaphorin V antibody
    • Semaphorin-3B antibody
    • Semaphorin-V antibody
    see all

Images

  • Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    The band observed at 50 kDa represents the secreted form of SEMA3B.

References

ab88489 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab88489.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up