Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13458(B)] to SEMA3D - C-terminal
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-SEMA3D antibody [EPR13458(B)] - C-terminal
See all SEMA3D primary antibodies
DescriptionRabbit monoclonal [EPR13458(B)] to SEMA3D - C-terminal
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
within Human SEMA3D aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O95025
- MCF7 cells, 293T, MCF7, fetal brain and fetal heart lysates.
This product was previously labelled as Semaphorin 3D
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Predicted molecular weight: 90 kDa.|
FunctionInduces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
DomainStrong binding to neuropilin is mediated by the carboxy third of the protein.
- Information by UniProt
- Coll 2 antibody
- Coll2 antibody
- Collapsin 2 antibody
Flow cytometric analysis of permeabilized MCF7 cells labeling SEMA3D using ab180174 at 1/10 (red) or a rabbit IgG (negative) (green).
All lanes : Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (ab180174) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Fetal brain lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
ab180174 has not yet been referenced specifically in any publications.