Overview

  • Product name

    Anti-SEMA3D antibody [EPR13458(B)] - C-terminal
    See all SEMA3D primary antibodies
  • Description

    Rabbit monoclonal [EPR13458(B)] to SEMA3D - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human SEMA3D aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O95025

  • Positive control

    • MCF7 cells, 293T, MCF7, fetal brain and fetal heart lysates.
  • General notes

     This product was previously labelled as Semaphorin 3D

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
    • Sequence similarities

      Belongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 PSI domain.
      Contains 1 Sema domain.
    • Domain

      Strong binding to neuropilin is mediated by the carboxy third of the protein.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Coll 2 antibody
      • Coll2 antibody
      • Collapsin 2 antibody
      • SEM3D_HUMAN antibody
      • Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3D antibody
      • Sema Z2 antibody
      • Sema3d antibody
      • Semaphorin-3D antibody
      • Semaphorin3D antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized MCF7 cells labeling SEMA3D using ab180174 at 1/10 (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (ab180174) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 90 kDa

    References

    ab180174 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180174.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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