Overview

  • Product name

  • Description

    Rabbit polyclonal to SEMA4F
  • Host species

    Rabbit
  • Specificity

    ab86549 recognizes isoform 2.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (VPRSVPRTSL PISEADSCLT RFAVPHTYNY SVLLVDPASH TLYVGARDTI) of human SEMA4F (AAH18361; O95754-2).

  • Positive control

    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-SEMA4F antibody (ab86549) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 66 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

References

ab86549 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. Because we carry over 70,000 products, I am sorry it is regrettably not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again by replying to this email prior to purchase for more information. Otherwise, we would be pleased if you wish to consider submitting an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

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