Anti-SEMA5A antibody (ab127002)
Key features and details
- Rabbit polyclonal to SEMA5A
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SEMA5A antibody
See all SEMA5A primary antibodies -
Description
Rabbit polyclonal to SEMA5A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SEMA5A aa 700-960.
Database link: Q13591 -
Positive control
- Mouse brain whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 121 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 121 kDa. |
Target
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Relevance
Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met. -
Cellular localization
Membrane; single-pass type I membrane protein -
Database links
- Entrez Gene: 9037 Human
- Entrez Gene: 20356 Mouse
- Omim: 609297 Human
- SwissProt: Q13591 Human
- SwissProt: Q62217 Mouse
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Alternative names
- SEMA5A antibody
- SEMAF antibody
- Semaphorin F antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab127002 has been referenced in 3 publications.
- Zhang S et al. Tumor-derived extracellular vesicles confer 5-fluorouracil resistance in esophageal cancer via long noncoding RNA AC116025.2 delivery. Mol Carcinog 61:1177-1190 (2022). PubMed: 36239547
- Tejedor G et al. MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis. Front Cell Dev Biol 9:579951 (2021). PubMed: 33738280
- Wang L et al. Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression. Aging (Albany NY) 12:21674-21686 (2020). PubMed: 33176280