Anti-SEMA5A antibody (ab51957)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-SEMA5A antibody
See all SEMA5A primary antibodies -
Description
Goat polyclonal to SEMA5A -
Host species
Goat -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Chicken, Human
Predicted to work with: Mouse, Rat, Cow, Dog
-
Immunogen
Synthetic peptide corresponding to SEMA5A aa 91-110 (internal sequence).
Sequence:DEATKKACYSKGKS
-
Positive control
- Human Colon, Heart and Kidney lysates
-
General notes
This product was previously labelled as Semaphorin 5A
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51957 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 4 - 6 µg/ml. | |
| IHC-Fr | 1/200. |
Target
-
Relevance
Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met. -
Cellular localization
Membrane; single-pass type I membrane protein -
Database links
- Entrez Gene: 9037 Human
- Entrez Gene: 20356 Mouse
- Omim: 609297 Human
- SwissProt: Q13591 Human
- SwissProt: Q62217 Mouse
-
Alternative names
- SEMA5A antibody
- SEMAF antibody
- Semaphorin F antibody
Images
-
Immunohistochemistry (Frozen sections) - Anti-SEMA5A antibody (ab51957)This image is courtesy of an Abreview submitted by Mrs Elena Astaninaab51957 (1/200) detecting SEMA5A in Chicken E11 embryo. Tissue was fixed in paraformaldehyde, permeabilised with 0.2% Triton X-100/PBS and blocked in 5% serum for 1 hour at 37oC. Incubation with primary antibody was performed for 16 hours in 0,5% BSA/ 2,5% serum/ PBS at 4oC. Please refer to abreview for further experimental details.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (ab51957)ab51957 at 4µg/ml staining SEMA5A in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was detected by HRP-staining.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Monticone M et al. Identification of a novel set of genes reflecting different in vivo invasive patterns of human GBM cells. BMC Cancer 12:358 (2012). ICC/IF ; Human . Read more (PubMed: 22901239) »
