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    sema5a-antibody-ab51957.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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Anti-SEMA5A antibody (ab51957)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (ab51957)

    Key features and details

    • Goat polyclonal to SEMA5A
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    Protein
    Recombinant Human SEMA5A protein (ab151658)

    View more associated products

    Overview

    • Product name

      Anti-SEMA5A antibody
      See all SEMA5A primary antibodies
    • Description

      Goat polyclonal to SEMA5A
    • Host species

      Goat
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Dog
    • Immunogen

      Synthetic peptide corresponding to SEMA5A aa 91-110 (internal sequence).
      Sequence:

      DEATKKACYSKGKS

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

       This product was previously labelled as Semaphorin 5A

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab51957 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Growth and Development
      • Axonal Guidance Proteins
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Neuroscience
      • Neurology process
      • Neuroregeneration
      • Neuroregeneration

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human SEMA5A protein (ab151658)

    Applications

    Our Abpromise guarantee covers the use of ab51957 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 4 - 6 µg/ml.

    Target

    • Relevance

      Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.
    • Cellular localization

      Membrane; single-pass type I membrane protein
    • Database links

      • Entrez Gene: 9037 Human
      • Entrez Gene: 20356 Mouse
      • Omim: 609297 Human
      • SwissProt: Q13591 Human
      • SwissProt: Q62217 Mouse
      • Alternative names

        • SEMA5A antibody
        • SEMAF antibody
        • Semaphorin F antibody

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (ab51957)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (ab51957)

        ab51957 at 4µg/ml staining SEMA5A in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was detected by HRP-staining.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab51957? Please let us know so that we can cite the reference in this datasheet.

      ab51957 has been referenced in 1 publication.

      • Monticone M  et al. Identification of a novel set of genes reflecting different in vivo invasive patterns of human GBM cells. BMC Cancer 12:358 (2012). ICC/IF ; Human . PubMed: 22901239

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-Semaphorin 5A antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Ovary/ Uterus/ Adrenal gland)
      Permeabilization
      Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
      Specification
      Ovary/ Uterus/ Adrenal gland
      Blocking step
      3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 03 2015

      Immunohistochemistry (Frozen sections) abreview for Anti-Semaphorin 5A antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Chicken Tissue sections (Chick embryo E11)
      Specification
      Chick embryo E11
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0,2% Triton X-100 in PBS, 10'
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Mrs. Elena Astanina

      Verified customer

      Submitted Jun 02 2008

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