Overview

  • Product name
  • Description
    Rabbit polyclonal to Sema6A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 247-534 of Human Sema6A (UniProt Q9H2E6).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and mouse brain lysates; NCI-N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 114 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.
  • Sequence similarities
    Belongs to the semaphorin family.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HT018 antibody
    • SEM6A_HUMAN antibody
    • SEMA antibody
    • Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A antibody
    • Sema Q antibody
    • Sema VIA antibody
    • SEMA6A 1 antibody
    • Sema6a antibody
    • SEMA6A-1 antibody
    • SEMA6A1 antibody
    • Semaphorin 6A 1 antibody
    • Semaphorin Q antibody
    • Semaphorin VIA antibody
    • Semaphorin-6A antibody
    • Semaphorin-6A-1 antibody
    • SEMAQ antibody
    • VIA antibody
    see all

Images

  • All lanes : Anti-Sema6A antibody (ab154938) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Hep G2 whole cell lysate
    Lane 3 : Raji whole lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 114 kDa



    7.5% SDS PAGE
  • Anti-Sema6A antibody (ab154938) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 114 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling Sema6A with ab154938 at 1/100 dilution.

References

ab154938 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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