1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol
using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.
Belongs to the semaphorin family. Contains 1 PSI domain. Contains 1 Sema domain.