Key features and details
- Rabbit polyclonal to SEMA6D
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEMA6D antibody
See all SEMA6D primary antibodies
DescriptionRabbit polyclonal to SEMA6D
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Orangutan
- ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
RelevanceSEMA6D belongs to the semaphorin family of proteins, which are a large family of secreted and transmembrane proteins, some of which function as repellent signals during axon guidance. SEMA6D shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro and may be a stop signal for DRG neurons in their target areas, and possibly also for other neurons. It may also be involved in the maintenance and remodeling of neuronal connections.
Cellular localizationIsoforms 1-6: Cell membrane; Single-pass type I membrane protein. Isoform 7: Cytoplasm.
- KIAA1479 antibody
- Q8NFY4 antibody
- Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D antibody
- Semaphorin 6D antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SEMA6D (green) using ab237693 at 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237693 has not yet been referenced specifically in any publications.