- Datasheet
- Specific References (23)
- Protocols
Overview
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Product nameAnti-Semaphorin 3A antibody
See all Semaphorin 3A primary antibodies -
DescriptionRabbit polyclonal to Semaphorin 3A
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
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Immunogen
Synthetic peptide (coupled to carrier protein):
RDGPNYQWVPYQGR
, corresponding to amino acids 359-372 of Human Semaphorin 3A (Peptide available as ab42768). -
Positive control
- Rat brain lysate for SDS PAGE immunoblots.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was affinity purified.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab23393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 19443185 | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18574596 | |
| WB | 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).Can be blocked with Human Semaphorin 3A peptide (ab88818). | |
| ELISA | 1/2000. |
Target
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RelevanceOne family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
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Cellular localizationSecreted
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Database links
- Entrez Gene: 10371 Human
- Entrez Gene: 20346 Mouse
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q14563 Human
- SwissProt: O08665 Mouse
- SwissProt: Q63548 Rat
- Unigene: 252451 Human
see all -
Alternative names
- Coll 1 antibody
- Coll1 antibody
- Collapsin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (ab23393)Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-Semaphorin 3A antibody (ab23393)Lanes 1-2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3-4 : Anti-Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lanes 2 & 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Predicted band size: 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature. -
Western blot - Anti-Semaphorin 3A antibody (ab23393)This image is a courtesy of Anonymous AbreviewAnti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?)
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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Immunohistochemistry (Frozen sections) - Anti-Semaphorin 3A antibody (ab23393)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.ab23393 staining Semaphorin 3A in 16 µm thick sections of Apteronotus leptorhynchus brain, spinal cord by Immunohistochemistry (Frozen sections).
Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab23393 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution. -
Immunohistochemistry (Frozen sections) - Anti-Semaphorin 3A antibody (ab23393)Image from Okada A & Tomooka Y, Neuroscience Letters, Volume 516, Issue 2, 16 May 2012, Pages 259–264. Fig 2.; doi: 10.1016/j.neulet.2012.04.005. with permission from Elsevier.Immunohistochemical analysis of murine developing cerebral cortices, staining Semaphorin 3A with ab23393.
Protocols
References
This product has been referenced in:
- Rienks M et al. Sema3A promotes the resolution of cardiac inflammation after myocardial infarction. Basic Res Cardiol 112:42 (2017). WB . Read more (PubMed: 28540528) »
- Inoue-Torii A et al. The level of urinary semaphorin3A is associated with disease activity in patients with minimal change nephrotic syndrome. Int J Nephrol Renovasc Dis 10:167-174 (2017). Read more (PubMed: 28790860) »
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