Product nameAnti-Semaphorin 3A antibody
See all Semaphorin 3A primary antibodies
DescriptionRabbit polyclonal to Semaphorin 3A
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
- Rat brain lysate for SDS PAGE immunoblots.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was affinity purified.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab23393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19443185|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18574596|
|WB||1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).Can be blocked with Human Semaphorin 3A peptide (ab88818).|
RelevanceOne family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
- Coll 1 antibody
- Coll1 antibody
- Collapsin 1 antibody
Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Lanes 1-2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3-4 : Anti-Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lanes 2 & 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Predicted band size: 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ab23393 staining Semaphorin 3A in 16 µm thick sections of Apteronotus leptorhynchus brain, spinal cord by Immunohistochemistry (Frozen sections).
Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab23393 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
Immunohistochemical analysis of murine developing cerebral cortices, staining Semaphorin 3A with ab23393.
This product has been referenced in:
- Rienks M et al. Sema3A promotes the resolution of cardiac inflammation after myocardial infarction. Basic Res Cardiol 112:42 (2017). WB . Read more (PubMed: 28540528) »
- Inoue-Torii A et al. The level of urinary semaphorin3A is associated with disease activity in patients with minimal change nephrotic syndrome. Int J Nephrol Renovasc Dis 10:167-174 (2017). Read more (PubMed: 28790860) »