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    semaphorin-3a-antibody-ab23393.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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Anti-Semaphorin 3A antibody (ab23393)

  • Datasheet
  • SDS
Reviews (5)Q&A (6)References (41)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (ab23393)
  • Western blot - Anti-Semaphorin 3A antibody (ab23393)
  • Western blot - Anti-Semaphorin 3A antibody (ab23393)

Key features and details

  • Rabbit polyclonal to Semaphorin 3A
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Semaphorin 3A antibody [EPR19367] (ab199475)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Semaphorin 3A antibody
    See all Semaphorin 3A primary antibodies
  • Description

    Rabbit polyclonal to Semaphorin 3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide (coupled to carrier protein):

    RDGPNYQWVPYQGR

    , corresponding to amino acids 359-372 of Human Semaphorin 3A (Peptide available as ab42768).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was affinity purified.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Semaphorin 3A peptide (ab88818)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Semaphorin 3A protein (ab185410)

Applications

Our Abpromise guarantee covers the use of ab23393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).Can be blocked with Human Semaphorin 3A peptide (ab88818).

Target

  • Relevance

    One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 10371 Human
    • Entrez Gene: 20346 Mouse
    • Entrez Gene: 29751 Rat
    • Omim: 603961 Human
    • SwissProt: Q14563 Human
    • SwissProt: O08665 Mouse
    • SwissProt: Q63548 Rat
    • Unigene: 252451 Human
    • Unigene: 372039 Mouse
    • Unigene: 44410 Rat
    see all
  • Alternative names

    • Coll 1 antibody
    • Coll1 antibody
    • Collapsin 1 antibody
    • HH16 antibody
    • Hsema I antibody
    • Hsema III antibody
    • Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A antibody
    • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A antibody
    • Sema III antibody
    • SEMA1 antibody
    • SEMA1-PEN antibody
    • SEMA3A antibody
    • SEMAD antibody
    • SEMAIII antibody
    • SEMAL antibody
    • Semaphorin 3A precursor antibody
    • Semaphorin III antibody
    • semaphorin L antibody
    • Semaphorin like growth cone guidance homolog of chick collapsin antibody
    • Semaphorin-D antibody
    • Semaphorin3A antibody
    • SemD antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (ab23393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (ab23393)
    Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Western blot - Anti-Semaphorin 3A antibody (ab23393)
    Western blot - Anti-Semaphorin 3A antibody (ab23393)
    Lanes 1-2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
    Lanes 3-4 : Anti-Semaphorin 3A antibody (ab25999)

    Lane 1 : Neonatal rat brain
    Lanes 2 & 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
    Lane 3 : Neonatal rat brain

    Predicted band size: 89 kDa



    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
  • Western blot - Anti-Semaphorin 3A antibody (ab23393)
    Western blot - Anti-Semaphorin 3A antibody (ab23393)This image is a courtesy of Anonymous Abreview
    Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 89 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (41)

    Publishing research using ab23393? Please let us know so that we can cite the reference in this datasheet.

    ab23393 has been referenced in 41 publications.

    • Zhang D  et al. Repression of miR-142-3p alleviates psoriasis-like inflammation by repressing proliferation and promoting apoptosis of keratinocytes via targeting Sema3A. Mol Cell Probes N/A:101573 (2020). PubMed: 32325105
    • Daneshvar N  et al. Premature satellite cell activation before injury accelerates myogenesis and disrupts neuromuscular junction maturation in regenerating muscle. Am J Physiol Cell Physiol 319:C116-C128 (2020). PubMed: 32374678
    • Hossain MM  et al. PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice. PLoS One 14:e0221440 (2019). PubMed: 31430342
    • Zhao J  et al. Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer. Eur J Pharmacol 847:11-18 (2019). PubMed: 30639311
    • Boggio EM  et al. Inhibition of Semaphorin3A Promotes Ocular Dominance Plasticity in the Adult Rat Visual Cortex. Mol Neurobiol N/A:N/A (2019). PubMed: 30706367
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-Semaphorin 3A antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2013

    Question

    Dear technical support,

    I have a couple questions about the product # ab23393 (SEMA3A Antibody rabbit polyclonal)

    - We would like to use such molecule as a capture antibody in a sandwich ELISA. Do you have any information related to your product in this kind of assay (titration, suitability for sandwich ELISA, etc)?

    - We are working with human vitreous samples, and there is no actual data on expected concentrations of Sema3A in the patients. It would be very relevant for us to know the detection limit of this antibody (ideally in pg/mL) if possible.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2013

    Answer

    Hello and thank you for contacting us. This antibody have not been tested for sandwich ELISA with a paired antibody, nor has the detection limit of the antibody been tested. I am sorry I could not be more helpful in this case. Please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 09 2013

    Question

    Buenas tardes,
    Muchas gracias por el peptido, ya nos ha llegado.
    Por otro lado, cuando hablé por primera vez por tlf con vuestra compañera Ariana, me comentó que podríais enviarme una alicuota nueva del anticuerpo. Me gustaría saber si es posible, después de haber tenido tantos problemas y haber hecho tantas pruebas, que nos enviarais una alicuota nueva.
    Tambien me gustaría saber, si habria la posibilidad de que os enviaramos alguna muestra nuestra, para que vosotros la detectarais con anti-sema3A, ya que tal vez sea más efectivo que seguir haciendo pruebas.
    Otra cuestión es sobre la disgregación del tejido. Yo lo hago mediante el Buffer TLB, puede ser que otro buffer funcione mejor? y, por qué?
    Muchas gracias nuevamente.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2012

    Answer

    Thank you for your reply.
    My colleague is currently out of the office and I'm afraid my Spanish is not good enough to reply in this language.
    Unfortunately testing customers samples in our lab is not possible.
    We appreciate the time you have spent on these experiments and, as agreed, we are pleased to arrange a replacement with one unit of ab23393. The order number is: *****.
    To check the status of the order please contact our Customer Service team and reference this number.
    About the lysis buffer, I would recommend to use a RIPA buffer (Radio Immuno Precipitation Assay buffer):
    150 mM sodium chloride
    1.0% NP-40 or Triton X-100
    0.5% sodium deoxycholate
    0.1% SDS (sodium dodecyl sulphate)
    50 mM Tris, pH 8.0

    For more details about the choice of lysis buffer and Western Blot I would suggest to look at our recommended protocol available on our website:
    Protocols homepage: https://www.abcam.com/index.html?pageconfig=popular_protocols
    Western Blot: https://www.abcam.com/index.html?pageconfig=resource&rid=11375
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Aug 01 2012

    Question

    Buenos días,
    acabo de hablar con XXXX, comentándole los problemas que estoy teniendo a la hora de detectar semaphorin3A en extracción proteíca a partir de células de bazo, y sistema inmune procedente de ratón.
    Como le he comentado, obtengo bandas a distintos pesos moleculares, que no corresponden con las bandas esperadas para esta proteína.
    Os he intentado enviar un archivo power point en el que adjuntaba varias de las pruebas que he realizado en westernblot, siendo las dos últimas diapositivas, las resultados más recientes, sin embargo, he recibido un mail de que no se ha podido enviar el mail correctamente, debido a que sobrepasaba el tamaño permitido. De este modo, cuando querais, contactar conmigo, para que os pueda enviar las fotos por otro medio.
    Nos gustaría saber si se os plantea algun cambio en el protocolo de la técnica, o si sería posible disponer del péptido inmunógeno para utilizarlo como control positivo, o para bloquear la membrana con el mismo.
    Muchas gracias,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Ya me han llegado las imágenes, muchas gracias por haber insistido.
    Me confirman que este anticuerpo se ha usado en WB con lisados de cerebro de ratón a diluciones 1/250 y 1/1000. La incubación se ha llevado a cabo durante 2 horas a temperatura ambiente y quimioluminiscencia como sistema de detección.
    El péptido inmunógeno está disponible en nuestro catálogo:
    https://www.abcam.com/Semaphorin-3A-peptide-ab42768.html
    y si te interesa te lo puedo enviar de forma gratuita para que lo probéis, confiando en que os ayude a evaluar el resultado obtenido.
    Espero tu respuesta al respecto, y confío en que esta información sea de utilidad.

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Question

    Is there a minimum mass or concentration of antibody to be compatible with this kit? What is the expected recovery?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for your call today and for your patience.

    I have discussedthe purification kit ab102783with my colleagues, and the minimum amount of antibody that is recommended to use with the kit is 20 ug.Since there are 50 ug per vial of ab23393, this kit will be compatible, and youcan dilute the antibody to 100 uL(the minimum volume required).

    The protein A usedwiththis kit has a high affinity for rabbit IgG, so the expected recovery is greater than 95%. The IgG will be edulted in 200-300 uL of buffer, however, so it will be more dilute than the original concentration. The neutralisation buffer is not compatible with most BCA or Bradford reagents, so the purified concentration should be measured before addition of the neutraliser, or an OD280 should be used to measure concentration.

    I hope this information will be useful, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    Abreview testing codes have expires prematurely.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2011

    Answer

    Thank you for your call today, and I apologize for the trouble with these codes! I have created new codes which will expire on March 1, 2012. I will go through your Abreviews and put these new codes in our internal notes so that the discounts can be applied to your orders tomorrow. Please let me know if you have any questions, or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-Semaphorin 3A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
    Specification
    Brain, spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More

    Dr. Ruxandra Sirbulescu

    Verified customer

    Submitted Nov 14 2011

    Western blot abreview for Anti-Semaphorin 3A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (Brain)
    Loading amount
    15 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 07 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Semaphorin 3A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Intervertebral disc/articular cartilage and brain)
    Specification
    Intervertebral disc/articular cartilage and brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 1mg/ml trypsin 20 mins
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Semaphorin 3A antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain endothelial cells)
    Specification
    brain endothelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% PBS/Triton X100
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Esperanza Meléndez-Herrera

    Verified customer

    Submitted Aug 29 2008

    1-10 of 11 Abreviews or Q&A

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