Anti-Semaphorin 3c antibody (ab39300)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.

Overview

Properties

Applications

  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab39299).
    Not yet tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The Semaphorins constitute a large family of secreted and membrane-tethered cell signaling molecules. They have functions in neural development, immunology, cardiac growth, vascular development, lung morphogenesis and axial bone patterning. Semaphorins are defined by the presence of a conserved Sema domain at the N-terminus. These proteins can be classified into eight classes depending on their structure and species origin. Classes 3 through 7 are found in vertebrates; class 3 are secreted, class 7 are GPI-anchored and class 4 through 6 are transmembrane proteins. Two distinct transmembrane receptor families have been identified as Semaphorin receptors. Neuropilins provide binding specificity for the class 3 Semaphorins, whereas Plexins, which also contain a Sema domain serve as signaling partners for class 3 Semaphorins and as functional receptors for transmembrane Semaphorins. Semaphorin 3c inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is expressed maximally between days E10-12 with moderate levels from day E13 until birth.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Sema E antibody
      • SEMA3C antibody
      • semaE antibody
      • Semahorin3C antibody
      • Semaphorin E antibody
      • SemaphorinE antibody
      see all

    References

    ab39300 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
    Sample
    Mouse Cell (Neural Tube)
    Specification
    Neural Tube
    Permeabilization
    No
    Fixative
    IF-Paraformaldehyde/Paraffin embedded

    Abcam user community

    Verified customer

    Submitted Jun 13 2013

    Question
    Answer

    Thank you for your patience.

    I spoke to my colleague Jill who is in charge to source new proteins, and even she agrees that we should have this protein in our catalog, I am afraid this will take quite a while ( more than two month at least).

    Therefore, I am afraid we cannot help you with a full length protein you can use as a standard right now when you need it.

    I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for contacting us again.

    I think the problem with this peptide is that it will probably only be detected by the polyclonal. I cannot tell you if the monoclonal antibody can bind this peptide as the epitope wasn't mapped.

    However, I have asked our protein expert if it is possible to get our hands on the full length proteinand will let you know if I know anything more.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question
    Answer




    I am very pleased to hear you would like to accept our offer and test ab135167 Anti-Semaphorin 3c antibody [MM0607-29U11] in sELISA. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you for contacting us.

    Yes, that would be the way forward. Shall I send you the discount code, then?


    Looking forward to your reply.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you very much for your interest in our products.

    I am delighted to hear that you get on so well with this ab39300 Anti-Semaphorin 3c antibody. So far, as a fast track antibody, this product has only been tested in peptide ELISA, but not against the full, endogenousprotein.

    I would like to suggest to have a look at the ab135167 Anti-Semaphorin 3c antibody [MM0607-29U11]:To our knowledge, ab135167 has not been tested in sELISA. Therefore, I can offer a discount off a future purchase if you buy ab135167now, test it in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab135167 in sELISA I will then send a discount code. This code must be issued before purchasing ab135167 so please wait for my reply before ordering.

    2. Purchase ab135167 either by phone, fax, or online (www.abcam.com).

    3. Test it in sELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab135167 turns out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.


    Unfortunately, I cannot offer you any discounton the ab39300due to it's Fast Track nature. However, we would greatly appreciate your feedback on this antibody in both ELISA and IHC-P, whether positive or negative. We will award 950 bonus Abpoints to the first researcher who sends us positive feedback (including an image and details of materials and methods) on both methods. Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 bonus Abpoints. Just a little reminder: 700 Abpoints equal 150€ off your next order when you claim them.


    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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