Product nameAnti-Semaphorin 3E antibody
See all Semaphorin 3E primary antibodies
DescriptionGoat polyclonal to Semaphorin 3E
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- Human Prostate tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab112886 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab112886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.03 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 89 kDa). Preliminary experiments gave an approx 35kDa band in Human Prostate lysates. The 35kDa band was successfully blocked by incubation with the immunizing peptide.|
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Sema domain.
- Information by UniProt
- Coll 5 antibody
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This product has been referenced in:
- Mazzotta C et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther 17:221 (2015). IHC-P ; Human . Read more (PubMed: 26292963) »