Key features and details
- Rabbit polyclonal to Semaphorin 3F
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Semaphorin 3F antibody
DescriptionRabbit polyclonal to Semaphorin 3F
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide within Human Semaphorin 3F aa 735-785 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q13275
- Rat brain and spleen tissues
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
FunctionMay play a role in cell motility and cell adhesion.
Tissue specificityExpressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Sema domain.
Developmental stageDetected as early as embryonic day 10.
- Information by UniProt
- SEM3F_HUMAN antibody
- sema domain immunoglobulin domain Ig short basic domain secreted 3F antibody
- sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling Semaphorin 3F with ab203394 at 1/200 dilution, followed by secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat spleen tissue labeling Semaphorin 3F with ab203394 at 1/200 dilution, followed by secondary antibody and DAB staining.
ab203394 has been referenced in 1 publication.
- Poltavski DM et al. Venous endothelin modulates responsiveness of cardiac sympathetic axons to arterial semaphorin. Elife 8:N/A (2019). PubMed: 30735130