Overview

  • Product name

    Anti-Semaphorin 3F antibody
  • Description

    Rabbit polyclonal to Semaphorin 3F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Semaphorin 3F aa 735-785 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q13275

  • Positive control

    • Rat brain and spleen tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab203394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Function

    May play a role in cell motility and cell adhesion.
  • Tissue specificity

    Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Sema domain.
  • Developmental stage

    Detected as early as embryonic day 10.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • SEM3F_HUMAN antibody
    • sema domain immunoglobulin domain Ig short basic domain secreted 3F antibody
    • sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F antibody
    • Sema III/F antibody
    • Sema IV antibody
    • SEMA-IV antibody
    • SEMA3F antibody
    • SEMA4 antibody
    • SemaIII/F antibody
    • SemaIV antibody
    • SEMAK antibody
    • Semaphorin IV antibody
    • Semaphorin-3F antibody
    • Semaphorin3F antibody
    • SemaphorinIV antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling Semaphorin 3F with ab203394 at 1/200 dilution, followed by secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat spleen tissue labeling Semaphorin 3F with ab203394 at 1/200 dilution, followed by secondary antibody and DAB staining.

References

This product has been referenced in:

  • Poltavski DM  et al. Venous endothelin modulates responsiveness of cardiac sympathetic axons to arterial semaphorin. Elife 8:N/A (2019). Read more (PubMed: 30735130) »
See 1 Publication for this product

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