Overview

  • Product name

    Anti-Semaphorin 4D/CD100 antibody [A8]
    See all Semaphorin 4D/CD100 primary antibodies
  • Description

    Mouse monoclonal [A8] to Semaphorin 4D/CD100
  • Host species

    Mouse
  • Specificity

    This antibody recognises CD100 cell surface antigen
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity

    Reacts with: Human, Monkey, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human Semaphorin 4D/CD100.

  • General notes

     This product was previously labelled as Semaphorin 4D

     

Properties

Applications

Our Abpromise guarantee covers the use of ab33260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
WB
  • Application notes
    Flow Cyt: 1/1 (neat). Use 10µl of the suggested working dilution to label 106 cells in 100µl.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 96 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
    • Tissue specificity

      Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
    • Sequence similarities

      Belongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 PSI domain.
      Contains 1 Sema domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • A8 antibody
      • BB 18 antibody
      • BB18 antibody
      • CD 100 antibody
      • CD100 antibody
      • CD100 antigen antibody
      • Coll 4 antibody
      • COLL4 antibody
      • Collapsin 4 antibody
      • Collapsin4 antibody
      • GR3 antibody
      • Leukocyte activation antigen CD100 antibody
      • M sema G antibody
      • MSEMA antibody
      • SEM4D_HUMAN antibody
      • Sema 4d antibody
      • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D antibody
      • Sema H antibody
      • SEMA J antibody
      • Sema4d antibody
      • Semacl 2 antibody
      • Semacl2 antibody
      • SemaH antibody
      • SEMAJ antibody
      • Semaphorin 4D antibody
      • Semaphorin C like 2 antibody
      • Semaphorin H antibody
      • Semaphorin J antibody
      • Semaphorin-4D antibody
      • Semaphorin4D antibody
      • SemaphorinJ antibody
      • Semcl 2 antibody
      • Semcl2 antibody
      see all

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    TopBlock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3

    Abcam user community

    Verified customer

    Submitted Feb 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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