Key features and details
- Rabbit polyclonal to Semaphorin 4D/CD100
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Semaphorin 4D/CD100 antibody
See all Semaphorin 4D/CD100 primary antibodies
DescriptionRabbit polyclonal to Semaphorin 4D/CD100
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-tag) corresponding to Mouse Semaphorin 4D/CD100 aa 502-636. Expressed in E.coli.
FCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTS SCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKY GFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVS
Database link: O09126
- WB: Recombinant mouse Semaphorin 4D/CD100 protein; mouse serum. IHC-P: Mouse testis tissue.
This product was previously labelled as Semaphorin 4D
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 96 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Tissue specificityStrongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
- Information by UniProt
- A8 antibody
- BB 18 antibody
- BB18 antibody
Anti-Semaphorin 4D/CD100 antibody (ab231961) at 1 µg/ml + Recombinant mouse Semaphorin 4D/CD100 protein
Predicted band size: 96 kDa
Anti-Semaphorin 4D/CD100 antibody (ab231961) at 1 µg/ml + Mouse serum
Predicted band size: 96 kDa
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Semaphorin 4D/CD100 using ab231961 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231961 has been referenced in 1 publication.
- Liang J et al. Semaphoring 4D is required for the induction of antioxidant stress and anti-inflammatory effects of dihydromyricetin in colon cancer. Int Immunopharmacol 67:220-230 (2019). PubMed: 30562683