Product nameAnti-Semaphorin 4D/CD100 antibody [EP3569]
See all Semaphorin 4D/CD100 primary antibodies
DescriptionRabbit monoclonal [EP3569] to Semaphorin 4D/CD100
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human Semaphorin 4D/CD100. The exact sequence is proprietary.
- Jurkat cell lysate; Human spleen lysate; Human fetal kidney lysate; Paraffin embedded Human colon tissue; Paraffin embedded Human tonsil tissue; SH-SY5Y.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Semaphorin 4D
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 96 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMay play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Tissue specificityStrongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
Sequence similaritiesBelongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
- Information by UniProt
- A8 antibody
- BB 18 antibody
- BB18 antibody
Anti-Semaphorin 4D/CD100 antibody [EP3569] (ab134128) at 0.2 µg/ml (purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 120,150 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Semaphorin 4D/CD100 with Purified ab134128 at 1:250 dilution (4.8μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Semaphorin 4D/CD100 with Purified ab134128 at 1:120 dilution (10 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Semaphorin 4D/CD100 using ab134128 at 1/100 dilution. Positive staining of lymphocytes inclusion.
All lanes : Anti-Semaphorin 4D/CD100 antibody [EP3569] (ab134128) at 1/1000 dilution (unpurified)
Lane 1 : Camptothecin treated Jurkat cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human spleen lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 96 kDa
HRP labelled goat anti rabbit secondary antibody used at 1/2000 dilution
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling Semaphorin 4D/CD100 with ab134128 at 1/100 dilution
This product has been referenced in:
- Mishra P et al. ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming. J Clin Invest 128:323-340 (2018). Read more (PubMed: 29202474) »
- Mou P et al. Identification of a calmodulin-binding domain in Sema4D that regulates its exodomain shedding in platelets. Blood 121:4221-30 (2013). Read more (PubMed: 23564909) »