Overview

  • Product name

    Anti-Semaphorin 7a antibody
    See all Semaphorin 7a primary antibodies
  • Description

    Rabbit polyclonal to Semaphorin 7a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal amino acids 520-569 (SIYSSERSVL QSINPAEPHK ECPNPKPDKA PLQKVSLAPN SRYYLSCPME) of Human Semaphorin 7a (NP_003603).

  • Positive control

    • MDA-MB-435S cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab133803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa.

Target

  • Function

    Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
  • Tissue specificity

    Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney.
  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localization

    Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 108 antibody
    • CD108 antibody
    • CD108 antigen antibody
    • CDw108 antibody
    • H Sema K1 antibody
    • H Sema L antibody
    • H-SEMA-K1 antibody
    • H-Sema-L antibody
    • JMH antibody
    • JMH blood group antigen antibody
    • John Milton Hagen blood group H Sema K1 antibody
    • John Milton Hargen human blood group Ag antibody
    • John-Milton-Hargen human blood group Ag antibody
    • MGC126692 antibody
    • MGC126696 antibody
    • SEM7A_HUMAN antibody
    • Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A antibody
    • Sema K1 antibody
    • Sema L antibody
    • SEMA7A antibody
    • SEMAK1 antibody
    • SEMAL antibody
    • Semaphorin 7A GPI membrane anchor antibody
    • Semaphorin K1 antibody
    • Semaphorin L antibody
    • Semaphorin-7A antibody
    • Semaphorin-K1 antibody
    • Semaphorin-L antibody
    • Semaphorin7a antibody
    see all

Images

  • Anti-Semaphorin 7a antibody (ab133803) at 1 µg/ml + MDA-MB-435S cell lysate at 10 µg

    Predicted band size: 75 kDa

References

ab133803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Traci Lyons

Verified customer

Submitted Aug 23 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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