Anti-Semaphorin 7a antibody [MEM-150] (ab8222)

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Key features and details

  • Mouse monoclonal [MEM-150] to Semaphorin 7a
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Semaphorin 7a antibody [MEM-150]
    See all Semaphorin 7a primary antibodies

  • Description

    Mouse monoclonal [MEM-150] to Semaphorin 7a

  • Host species

    Mouse

  • Specificity

    Human CD108 (JMH blood group antigen).

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue/ cell preparation (Human). (T cell line HPB-ALL).

Properties

Applications

Our Abpromise guarantee covers the use of ab8222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 4 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non-reducing conditions.

Target

  • Function

    Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

  • Tissue specificity

    Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney.

  • Sequence similarities

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.

  • Cellular localization

    Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 108 antibody
      • CD108 antibody
      • CD108 antigen antibody
      • CDw108 antibody
      • H Sema K1 antibody
      • H Sema L antibody
      • H-SEMA-K1 antibody
      • H-Sema-L antibody
      • JMH antibody
      • JMH blood group antigen antibody
      • John Milton Hagen blood group H Sema K1 antibody
      • John Milton Hargen human blood group Ag antibody
      • John-Milton-Hargen human blood group Ag antibody
      • MGC126692 antibody
      • MGC126696 antibody
      • SEM7A_HUMAN antibody
      • Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A antibody
      • Sema K1 antibody
      • Sema L antibody
      • SEMA7A antibody
      • SEMAK1 antibody
      • SEMAL antibody
      • Semaphorin 7A GPI membrane anchor antibody
      • Semaphorin K1 antibody
      • Semaphorin L antibody
      • Semaphorin-7A antibody
      • Semaphorin-K1 antibody
      • Semaphorin-L antibody
      • Semaphorin7a antibody
      see all

    Images

    • Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)
      Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)
      Flow cytometry of human peripheral blood cells with ab8222 at 3 µg/ml

    References

    Publishing research using ab8222? Please let us know so that we can cite the reference in this datasheet.

    ab8222 has been referenced in 3 publications.

    • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . PubMed: 22634564
    • Angelisova P  et al. Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology 200:234-45 (1999). PubMed: 10416131
    • Mudad R  et al. Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion 35:566-70 (1995). PubMed: 7631388

    Customer reviews and Q&As

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    Question

    Your datasheet lists this antibody as an IgG1 subclass. However, the monoclonal produced by MEM-150 is described elsewhere as an IgM. Please clarify this discrepancy and confirm the subclass of this antibody. Thanks! Patty Tam

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    Answer

    You are indeed correct. MEM-150 produces an IgM antibody. We are grateful that you brought it to our attention. Many thanks.

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