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  1. Link

    semaphorin-7a-antibody-mem-150-ab8222.pdf

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Cardiovascular Blood Blood Cell Antigens
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Anti-Semaphorin 7a antibody [MEM-150] (ab8222)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (4)

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Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)

    Key features and details

    • Mouse monoclonal [MEM-150] to Semaphorin 7a
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgM

    Conjugates logo Related conjugates and formulations

    FITC

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    Overview

    • Product name

      Anti-Semaphorin 7a antibody [MEM-150]
      See all Semaphorin 7a primary antibodies
    • Description

      Mouse monoclonal [MEM-150] to Semaphorin 7a
    • Host species

      Mouse
    • Specificity

      Human CD108 (JMH blood group antigen).
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human Semaphorin 7a. T cell line HPB-ALL

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 8.0
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Proprietary Purification
    • Purification notes

      Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
    • Clonality

      Monoclonal
    • Clone number

      MEM-150
    • Myeloma

      unknown
    • Isotype

      IgM
    • Light chain type

      unknown
    • Research areas

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • Cardiovascular
      • Blood
      • Other
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Neuroscience
      • Neurology process
      • Growth and Development
      • Axonal Guidance Proteins
      • Neuroscience
      • Neurology process
      • Neurogenesis

    Associated products

    • Alternative Versions

      • FITC Anti-Semaphorin 7a antibody [MEM-150] (ab26012)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      • Goat Anti-Mouse IgM H&L (ab9167)
      • Rabbit Anti-Mouse IgM mu chain (ab9175)
    • Isotype control

      • Mouse IgM [B11/7] - Isotype control (ab91545)
    • Recombinant Protein

      • Recombinant Human Semaphorin 7a protein (Fc Chimera His Tag) (ab227401)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab8222 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 4 µg/ml.

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    Notes
    Flow Cyt
    Use a concentration of 4 µg/ml.

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
    • Tissue specificity

      Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney.
    • Sequence similarities

      Belongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 PSI domain.
      Contains 1 Sema domain.
    • Cellular localization

      Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.
    • Target information above from: UniProt accession O75326 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8482 Human
      • Omim: 607961 Human
      • SwissProt: O75326 Human
      • Unigene: 24640 Human
      • Alternative names

        • CD 108 antibody
        • CD108 antibody
        • CD108 antigen antibody
        • CDw108 antibody
        • H Sema K1 antibody
        • H Sema L antibody
        • H-SEMA-K1 antibody
        • H-Sema-L antibody
        • JMH antibody
        • JMH blood group antigen antibody
        • John Milton Hagen blood group H Sema K1 antibody
        • John Milton Hargen human blood group Ag antibody
        • John-Milton-Hargen human blood group Ag antibody
        • MGC126692 antibody
        • MGC126696 antibody
        • SEM7A_HUMAN antibody
        • Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A antibody
        • Sema K1 antibody
        • Sema L antibody
        • SEMA7A antibody
        • SEMAK1 antibody
        • SEMAL antibody
        • Semaphorin 7A GPI membrane anchor antibody
        • Semaphorin K1 antibody
        • Semaphorin L antibody
        • Semaphorin-7A antibody
        • Semaphorin-K1 antibody
        • Semaphorin-L antibody
        • Semaphorin7a antibody
        see all

      Images

      • Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)
        Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)
        Flow cytometry of human peripheral blood cells with ab8222 at 3 µg/ml

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab8222? Please let us know so that we can cite the reference in this datasheet.

      ab8222 has been referenced in 4 publications.

      • Zhao Z  et al. LncRNA DLX6-AS1 Promotes Malignant Phenotype and Lymph Node Metastasis in Prostate Cancer by Inducing LARGE Methylation. Front Oncol 10:1172 (2020). PubMed: 32850336
      • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . PubMed: 22634564
      • Angelisova P  et al. Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology 200:234-45 (1999). PubMed: 10416131
      • Mudad R  et al. Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion 35:566-70 (1995). PubMed: 7631388

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Your datasheet lists this antibody as an IgG1 subclass. However, the monoclonal produced by MEM-150 is described elsewhere as an IgM. Please clarify this discrepancy and confirm the subclass of this antibody. Thanks! Patty Tam

      Read More

      Abcam community

      Verified customer

      Asked on Mar 01 2002

      Answer

      You are indeed correct. MEM-150 produces an IgM antibody. We are grateful that you brought it to our attention. Many thanks.

      Read More

      Abcam Scientific Support

      Answered on Mar 04 2002

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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