Anti-Semaphorin 7a antibody [MEM-150] (ab8222)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Semaphorin 7a antibody [MEM-150]
See all Semaphorin 7a primary antibodies -
Description
Mouse monoclonal [MEM-150] to Semaphorin 7a -
Host species
Mouse -
Specificity
Human CD108 (JMH blood group antigen). -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human). (T cell line HPB-ALL).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-150 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab8222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 22896629 | |
| Flow Cyt | Use a concentration of 4 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Use under non-reducing conditions. |
Target
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Function
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
Tissue specificity
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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Database links
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
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Alternative names
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
Images
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![Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)](https://www.abcam.com/ps/products/8/ab8222/Images/ab8222-331846-anti-semaphorin-7a-antibody-mem-150-flow-cytometry.jpg)
Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)Flow cytometry of human peripheral blood cells with ab8222 at 3 µg/ml
Protocols
References
This product has been referenced in:
- Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »
- Angelisova P et al. Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology 200:234-45 (1999). Read more (PubMed: 10416131) »
