Recombinant
RabMAb

Recombinant Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

Overview

  • Product name

    Anti-Semenogelin I antibody [EPR3675(2)]
    See all Semenogelin I primary antibodies
  • Description

    Rabbit monoclonal [EPR3675(2)] to Semenogelin I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Semenogelin I aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Y79 and Human fetal lung lysates; Human seminal vesicle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3675(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
      Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
    • Tissue specificity

      Seminal vesicle.
    • Sequence similarities

      Belongs to the semenogelin family.
    • Post-translational
      modifications

      Transglutaminase substrate.
      Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha-Inhibin-31 antibody
      • Alpha-Inhibin-92 antibody
      • Cancer/testis antigen 103 antibody
      • CT103 antibody
      • dJ172H20.2 (semenogelin I) antibody
      • dJ172H20.2 antibody
      • MGC14719 antibody
      • Semen coagulating protein antibody
      • Semenogelin antibody
      • Semenogelin I antibody
      • SEMG antibody
      • SEMG1 antibody
      • SEMG1_HUMAN antibody
      • Seminal basic protein antibody
      • seminal vesicle secretory protein 5 antibody
      • SgI antibody
      • Svp-1 antibody
      • Svp5 antibody
      • SVPIIA antibody
      • Svs2 antibody
      • SVS2P antibody
      • Svs2p2 antibody
      • Svs5 antibody
      see all

    Images

    • All lanes : Anti-Semenogelin I antibody [EPR3675(2)] (ab139405) at 1/1000 dilution

      Lane 1 : Y79 lysate
      Lane 2 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunohistochemical analysis of paraffin-embedded Human seminal vesicle tissue labelling Semenogelin I with ab139405 antibody at 1/50 dilution.

    References

    This product has been referenced in:

    • Zhou QZ  et al. Expressions of miR-525-3p and its target gene SEMG1 in the spermatozoa of patients with asthenozoospermia. Andrology 7:220-227 (2019). Read more (PubMed: 30575326) »
    • Li S  et al. Crucial role of estrogen for the mammalian female in regulating semen coagulation and liquefaction in vivo. PLoS Genet 13:e1006743 (2017). WB ; Mouse . Read more (PubMed: 28414719) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your reply.

    It may indeed be easier to try this experiment with the rabbit monoclonal antibody ab139405. However, the immunogen information is proprietary information. In order to try to help you further could you explain what you are trying to achieve?

    Is the target of Semenogelin I important?

    What do you intend to use the peptide displacement experiment for?

    Do you intend to publish the results of this experiment?

    I hope with this information I may be able to provide further suggestions as to how this experiment may be designed.

    I look forward to receiving your reply.

    Read More

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