Recombinant Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3675(2)] to Semenogelin I
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Semenogelin I antibody [EPR3675(2)]
See all Semenogelin I primary antibodies -
Description
Rabbit monoclonal [EPR3675(2)] to Semenogelin I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Semenogelin I aa 50-150. The exact sequence is proprietary.
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Positive control
- Y79 and Human fetal lung lysates; Human seminal vesicle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3675(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone. -
Tissue specificity
Seminal vesicle. -
Sequence similarities
Belongs to the semenogelin family. -
Post-translational
modificationsTransglutaminase substrate.
Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6406 Human
- Entrez Gene: 20944 Mouse
- Entrez Gene: 25013 Rat
- Omim: 182140 Human
- SwissProt: P04279 Human
- Unigene: 1968 Human
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Alternative names
- Alpha-Inhibin-31 antibody
- Alpha-Inhibin-92 antibody
- Cancer/testis antigen 103 antibody
see all
Images
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All lanes : Anti-Semenogelin I antibody [EPR3675(2)] (ab139405) at 1/1000 dilution
Lane 1 : Y79 lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
Immunohistochemical analysis of paraffin-embedded Human seminal vesicle tissue labelling Semenogelin I with ab139405 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab139405 has been referenced in 2 publications.
- Zhou QZ et al. Expressions of miR-525-3p and its target gene SEMG1 in the spermatozoa of patients with asthenozoospermia. Andrology 7:220-227 (2019). PubMed: 30575326
- Li S et al. Crucial role of estrogen for the mammalian female in regulating semen coagulation and liquefaction in vivo. PLoS Genet 13:e1006743 (2017). WB ; Mouse . PubMed: 28414719