RecombinantRabMAb

Recombinant Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

Submit a review Q&A (1)References (2)
Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
  • Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3675(2)] to Semenogelin I
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Semenogelin I antibody [EPR3675(2)]
    See all Semenogelin I primary antibodies

  • Description

    Rabbit monoclonal [EPR3675(2)] to Semenogelin I

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Semenogelin I aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Y79 and Human fetal lung lysates; Human seminal vesicle tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3675(2)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
      Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.

    • Tissue specificity

      Seminal vesicle.

    • Sequence similarities

      Belongs to the semenogelin family.

    • Post-translational
      modifications

      Transglutaminase substrate.
      Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Alpha-Inhibin-31 antibody
        • Alpha-Inhibin-92 antibody
        • Cancer/testis antigen 103 antibody
        • CT103 antibody
        • dJ172H20.2 (semenogelin I) antibody
        • dJ172H20.2 antibody
        • MGC14719 antibody
        • Semen coagulating protein antibody
        • Semenogelin antibody
        • Semenogelin I antibody
        • SEMG antibody
        • SEMG1 antibody
        • SEMG1_HUMAN antibody
        • Seminal basic protein antibody
        • seminal vesicle secretory protein 5 antibody
        • SgI antibody
        • Svp-1 antibody
        • Svp5 antibody
        • SVPIIA antibody
        • Svs2 antibody
        • SVS2P antibody
        • Svs2p2 antibody
        • Svs5 antibody
        see all

      Images

      • Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
        Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
        All lanes : Anti-Semenogelin I antibody [EPR3675(2)] (ab139405) at 1/1000 dilution

        Lane 1 : Y79 lysate
        Lane 2 : Human fetal lung lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 52 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

        Immunohistochemical analysis of paraffin-embedded Human seminal vesicle tissue labelling Semenogelin I with ab139405 antibody at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)
        Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

      References (2)

      Publishing research using ab139405? Please let us know so that we can cite the reference in this datasheet.

      ab139405 has been referenced in 2 publications.

      • Zhou QZ  et al. Expressions of miR-525-3p and its target gene SEMG1 in the spermatozoa of patients with asthenozoospermia. Andrology 7:220-227 (2019). PubMed: 30575326
      • Li S  et al. Crucial role of estrogen for the mammalian female in regulating semen coagulation and liquefaction in vivo. PLoS Genet 13:e1006743 (2017). WB ; Mouse . PubMed: 28414719

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Dear xxxxx,

      Thanks for the quick reply. That is a good point. The reason that I selected that antibody is that you list the synthetic immunogen sequence on the product page.

      In that case would your monoclonal Anti-Semenogelin I antibody [EPR3675(2)] (ab139405) be more likely to perform this task? And if so, do you know the sequence of its synthetic peptide immunogen?

      Thanks,

      Anti-Semenogelin I antibody [EPR3675(2)] (ab139405)

      Read More
      Answer

      Thank you for your reply.

      It may indeed be easier to try this experiment with the rabbit monoclonal antibody ab139405. However, the immunogen information is proprietary information. In order to try to help you further could you explain what you are trying to achieve?

      Is the target of Semenogelin I important?

      What do you intend to use the peptide displacement experiment for?

      Do you intend to publish the results of this experiment?

      I hope with this information I may be able to provide further suggestions as to how this experiment may be designed.

      I look forward to receiving your reply.

      Read More

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