Recombinant Anti-Senataxin antibody [BLR050F] - BSA free (ab243904)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR050F] to Senataxin - BSA free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Senataxin antibody [BLR050F] - BSA free
See all Senataxin primary antibodies -
Description
Rabbit monoclonal [BLR050F] to Senataxin - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Senataxin aa 900-950. The exact sequence is proprietary. NP_055861.3 and Gene ID 23064.
Database link: Q7Z333 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR050F -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
|
IP |
Use at an assay dependent concentration.
Use 6µl/mg lysate. |
Notes |
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WB
1/1000. |
IP
Use at an assay dependent concentration. Use 6µl/mg lysate. |
Target
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Function
Probable helicase, which may be involved in RNA maturation (By similarity). Involved in DNA double-strand breaks damage response generated by oxidative stress. -
Tissue specificity
Highly expressed in skeletal muscle. Expressed in heart, fibroblast, placenta and liver. Weakly expressed in brain and lung. Expressed in the cortex of the kidney (highly expressed in tubular epithelial cells but low expression in the glomerulus). -
Involvement in disease
Defects in SETX are the cause of spinocerebellar ataxia autosomal recessive type 1 (SCAR1) [MIM:606002]; also known as ataxia-ocular apraxia 2. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR1 is an autosomal recessive form associated with peripheral neuropathy and elevated serum alpha-fetoprotein, immunoglobulins and, less commonly, creatine kinase levels. Some SCAR1 patients manifest oculomotor apraxia.
Defects in SETX are a cause of amyotrophic lateral sclerosis type 4 (ALS4) [MIM:602433]. ALS4 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. ALS4 is a childhood- or adolescent-onset form characterized by slow disease progression and the sparing of bulbar and respiratory muscles. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. Cytoplasm. May be detected in the nucleolus only in cycling cells (By similarity). Most abundant in the nucleus. Detected in granules. Colocalized in cycling cells with FBL in the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 23064 Human
- Omim: 608465 Human
- SwissProt: Q7Z333 Human
- Unigene: 460317 Human
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Alternative names
- ALS4 antibody
- Amyotrophic lateral sclerosis 4 protein antibody
- AOA2 antibody
see all
Images
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Senataxin was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243904 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243904 at 1/1000 dilution.
Lane 1: ab243904 IP in HeLa cell lysate.
Lane 2: Control IgG in HeLa cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot analysis using ab243904 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW 620 whole cell lysate (50 µg).
Lane 7: SK-MEL-28 whole cell lysate (50 µg).
Lane 8: Jurkat whole cell lysate (50 µg).
Lane 9: K562 whole cell lysate (50 µg).
Lane 10: RPMI 8226 whole cell lysate (50 µg).
Lane 11: 786-O whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243904 has been referenced in 1 publication.
- Saha S et al. Resolution of R-loops by topoisomerase III-β (TOP3B) in coordination with the DEAD-box helicase DDX5. Cell Rep 40:111067 (2022). PubMed: 35830799