Overview

  • Product name
    Senescence Detection Kit
  • Sample type
    Tissue, Adherent cells
  • Assay type
    Enzyme activity
  • Product overview

    Senescence Detection Kit (ab65351) is designed to histochemically detect SA-beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of beta-galactosidase (SA-beta-Gal) activity, and altered patterns of gene expression.

  • Tested applications
    Suitable for: IHC-Frmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab65351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Frozen sections) - Senescence Detection Kit (ab65351)
    Immunohistochemistry (Frozen sections) - Senescence Detection Kit (ab65351)
    Senescence-associated beta-galactosidase staining at the 2nd(A), 4th(B), 8th(C), 12th passage in vitro expansion. Cells were plated at a density of 10,000 cells/cm2 for 24h before staining. Five representative images (100x) were taken from diverse areas of cell culture, using phase-contrast microscopy to assess the number of positive cells.

    Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8:18

Protocols

References

This product has been referenced in:

See all 25 Publications for this product

Publishing research using ab65351? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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27 reviews or Q&A

10
Votes: Highest First

The included protocol describes a method to stain cells in a 12-well plate. However, you also indicate that it is possible to use this assay on paraffin embedded samples on glass cover plates. What modifications to the protocol, if any, should be made ...

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I can confirm frozen tissue sections can be used with ab65351Senescence Detection Kit. The kit has been used for frozen skin sections successfully. You can either fix the frozen tissue in the supplied fixation solution or use your preferred fixation of...

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I am interested in purchasing your Senescence Detection Kit (ab65351).

I saw that it works with frozen and paraffin sections – My question: can plastic sections be used with this kit, too?

Thank you very much,

I am sorry we have not tested plastic embedded tissue sections with this kit so we may not be able to provide you required details. You can however test this kit by keeping frozen sections as positive control.

Customer using rat and human SWAN cells. Laminin is necessary to adhere cells to TC plate. Would the laminin interfere with the assay?

Laminin should not interfere with the staining. The stain will bind only to SA-β-Gal. Also, you will have a normal control cell sample, which you would also grow in laminin and perform the same staining. Any blue color development observed in thes...

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Ich bin interessiert an ihrem "senescence-associated beta-Gal Assay" und wollte fragen ob Sie ein Protokoll hätten indem die Anwendung an humanen T Zellen (also Suspensionzellen) beschrieben wird.



ab65351 (Senescence Detection Kit) wurde nicht mit Suspensionszellen getestet oder dafür optimiert.

Da dieses Kit allerdings benutzt wird um fixierte Zellen anzufärben, könnte das folgende Protokoll vielleicht benutzt...

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Dear Team,

Hope this mail finds you well.

Kindly check on the below complaint where a broken vial was recieved by the customer in ab65351 supplied recently to them.

Thank you & Regards.

Thank you for your e-mail. I am sorry about this accident.

This is to confirm that I have been liaising with the Lab to get a new vial of the 100X Staining Supplement. We need to create a datasheet and get the product from the Lab. As soon as...

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do you have any data on testing at higher pH to reduce background?
have this kit actually been tested in paraffin embedded section? the reference is for frozen?

Thank you for contacting us.



Our development scientist has optimized this kit to work best at the conditions mentioned in our datasheet. But sometimes, you have to treat expts case by case and it is possible that in your case, the b...

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Thank you for your mail.

Please see below the details as desired by you:

Abcam Order Ref. No. 1182683

Looking forward towards your valued confirmation on it.

With Best Regards,

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your reply.

Dr. XXXX& we talked to this customer regarding her complaint for this kit which she used earlier & got positive results. But this time the kit is not working.

Please see the attached experimental data...

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Thank you for your email.

Could you provide the purchase order number? I will sending you a replacement in your next shipment as XXXX agreed.

Thanks!

Please find below a complaint from one of our customer to whom we supplied Senescence Detection Kit (ab65351) from our Abcam range.

Request you to kindly look into it & advise us accordingly to do further needful on it.

Looking f...

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Thank you for your email. I am sorry to hear that you are experiencing problems with lot GR79964-1.

We recommend warming the staining supplement as it is in 100X concentration and frozen. It is not unusual this to be observed jelly like due to...

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Inquiry: After SA-B-Gal staining (Senescence Detection Kit (ab65351)), can I dehydrate tissu section in order to use my mounting medium ? Thanks for your support.

Thank you for contacting us. Although we have never tried dehydrating our sections in order to mount them, I think it would be ok to do so, once you have confirmed the blue colour with the X-Gal staining

I hope this information is helpful to y...

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1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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