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    senescence-detection-kit-ab65351.pdf

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Senescence Detection Kit (ab65351)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (23)References (76)

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Human Wharton's jelly cells - Senescence Detection Kit (ab65351)

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Colorimetric
    • Assay time: 1 hr 10 min
    • Sample type: Adherent cells, Tissue

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    Overview

    • Product name

      Senescence Detection Kit
      See all Senescence-associated beta Galactosidase kits
    • Detection method

      Colorimetric
    • Sample type

      Tissue, Adherent cells
    • Assay type

      Enzyme activity
    • Assay time

      1h 10m
    • Product overview

      Senescence Detection Kit (ab65351) is designed to histochemically detect SA-beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.


      See Senescence Assay Kit ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.


      Senescence assay protocol summary:
      - wash cells / sections with PBS
      - fix with fixative solution for 10 min
      - wash with PBS
      - incubate in staining solution mix for 1 hr
      - analyze staining with a microscope

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K320 Senescence Detection Kit. K320-250 is the same size as the 250 test size of ab65351.

      Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of beta-galactosidase (SA-beta-Gal) activity, and altered patterns of gene expression.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Storage buffer

      Preservative: None
    • Components Identifier 250 tests 5000 tests
      100X Staining Supplement Red 1 x 1.5ml 20 x 1.5ml
      1X Fixative Solution NM 1 x 125ml 20 x 125ml
      1X Staining Solution WM 1 x 125ml 20 x 125ml
      X-Gal (150 mg, lyophilized) Green 1 vial 20 vials
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Cell Viability and Senescence Kits
      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • proliferation and senescence
      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • Cell Damage
    • Relevance

      Cellular senescence is a growth-arrest program by which normal diploid cells lose the ability to divide, and it plays a critical role in regulating lifespan both in vivo and in vitro. Cellular senescence occurs as reflection of organism aging and in response to internal and external stress signals.

    Images

    • Human Wharton's jelly cells - Senescence Detection Kit (ab65351)
      Human Wharton's jelly cells - Senescence Detection Kit (ab65351)Angelucci S et al., Proteome Sci, 8, 18, 2010 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0
      Senescence-associated beta-galactosidase staining at the 2nd(A), 4th(B), 8th(C), 12th passage in vitro expansion. Cells were plated at a density of 10,000 cells/cm2 for 24h before staining. Five representative images (100x) were taken from diverse areas of cell culture, using phase-contrast microscopy to assess the number of positive cells.

      Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8:18

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (76)

    Publishing research using ab65351? Please let us know so that we can cite the reference in this datasheet.

    ab65351 has been referenced in 76 publications.

    • Niklander SE  et al. The role of icIL-1RA in keratinocyte senescence and development of the senescence-associated secretory phenotype. J Cell Sci 134:N/A (2021). PubMed: 33526711
    • Kaur A  et al. WNT inhibition creates a BRCA-like state in Wnt-addicted cancer. EMBO Mol Med 13:e13349 (2021). PubMed: 33660437
    • Gao P  et al. MKL1 cooperates with p38MAPK to promote vascular senescence, inflammation, and abdominal aortic aneurysm. Redox Biol 41:101903 (2021). PubMed: 33667992
    • Belmans N  et al. In vitro Assessment of the DNA Damage Response in Dental Mesenchymal Stromal Cells Following Low Dose X-ray Exposure. Front Public Health 9:584484 (2021). PubMed: 33692980
    • Vannini E  et al. CTX-CNF1 Recombinant Protein Selectively Targets Glioma Cells In Vivo. Toxins (Basel) 13:N/A (2021). PubMed: 33800135
    View all Publications for this product

    Customer reviews and Q&As

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    Beta-galactosidase activity detection in dermal fibroblasts

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Senescence detection kit was used to detect beta-galactosidase activity in adherent primary dermal fibroblasts previously exposed to H2O2 (50 or 100 µM) for 5 days. 5 days post-treatment, cells were fixed using the fixative solution for 5 min only and stained using the staining solution at 37°C for 19-20 hours. Cells were observed using an inverted microscope with a focus of 70X. As soon as the senescence induction protocol was optimized, the senescence kit was rather easy to us but would be difficult to use for senescent cell quantification.

    DR. Agnès Tessier

    Verified customer

    Submitted Jan 30 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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