Overview

  • Product name

    Anti-SENP1 antibody [EPR3844] (HRP)
    See all SENP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3844] to SENP1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SENP1 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: HUVEC, Jurkat, Daudi and U87-MG whole cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab201814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Function

    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
  • Tissue specificity

    Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
  • Sequence similarities

    Belongs to the peptidase C48 family.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • SENP 1 antibody
    • SENP1 antibody
    • SENP1_HUMAN antibody
    • Sentrin specific protease 1 antibody
    • Sentrin-specific protease 1 antibody
    • Sentrin/SUMO specific protease 1 antibody
    • Sentrin/SUMO specific protease antibody
    • Sentrin/SUMO specific protease SENP 1 antibody
    • Sentrin/SUMO specific protease SENP1 antibody
    • Sentrin/SUMO-specific protease SENP1 antibody
    • SUMO1/sentrin specific peptidase 1 antibody
    • SUMO1/sentrin specific protease 1 antibody
    • SuPr 2 antibody
    • SuPr2 antibody
    see all

Images

  • All lanes : Anti-SENP1 antibody [EPR3844] (HRP) (ab201814) at 1/5000 dilution

    Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201814 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab201814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201814.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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