Product nameAnti-SENP2 antibody
See all SENP2 primary antibodies
DescriptionRabbit polyclonal to SENP2
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from an internal region of Human SENP2
- MDA-MB-435 cell extracts
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab58418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
Sequence similaritiesBelongs to the peptidase C48 family.
DomainThe N-terminus is necessary and sufficient for nuclear envelope targeting.
modificationsPolyubiquitinated; which leads to proteasomal degradation.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
- Information by UniProt
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All lanes : Anti-SENP2 antibody (ab58418) at 1/500 dilution
Lane 1 : MDA-MB-435 cell extract
Lane 2 : MDA-MB-435 cell extract with immunizing peptide
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ICC/IF image of ab58418 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Wang R et al. Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2. Oncotarget 8:70142-70155 (2017). Read more (PubMed: 29050267) »
- Snow JW et al. Sumoylation Regulates Interaction of FOG1 with C-terminal-binding Protein (CTBP). J Biol Chem 285:28064-75 (2010). WB ; Human . Read more (PubMed: 20587419) »