Key features and details
- Rabbit polyclonal to SENP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SENP2 antibody
See all SENP2 primary antibodies
DescriptionRabbit polyclonal to SENP2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide containing a sequence corresponding to a region within N terminal amino acids 1 and 32 of Human SENP2 (NP_067640).
- A431, H1299, HepG2, Raji cell lysates. 293T and Molt-4 whole cell lysates. A431 cells and Cal27 xenograft.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
Sequence similaritiesBelongs to the peptidase C48 family.
DomainThe N-terminus is necessary and sufficient for nuclear envelope targeting.
modificationsPolyubiquitinated; which leads to proteasomal degradation.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
- Information by UniProt
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All lanes : Anti-SENP2 antibody (ab96865) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : MOLT-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A431, using SENP2 antibody (ab96865) at 1/200 dilution. Lower image merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SENP2 antibody (ab96865) at 1/500 dilution.
ab96865 has been referenced in 1 publication.
- Wang Q et al. Regulation of the Expression of DAPK1 by SUMO Pathway. Biomolecules 9:N/A (2019). PubMed: 30999631