Recombinant Anti-SENP2 antibody [EPR4356(2)] (ab124724)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)](https://www.abcam.com/ps/products/124/ab124724/Images/ab124724-138544-anti-senp2-antibody-epr43562-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4356(2)] to SENP2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SENP2 antibody [EPR4356(2)]
See all SENP2 primary antibodies -
Description
Rabbit monoclonal [EPR4356(2)] to SENP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SENP2 aa 350-450. The exact sequence is proprietary.
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Positive control
- MDA-MB-435, NIH:OVCAR-3, and 293T cell lysates; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4356(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. -
Sequence similarities
Belongs to the peptidase C48 family. -
Domain
The N-terminus is necessary and sufficient for nuclear envelope targeting. -
Post-translational
modificationsPolyubiquitinated; which leads to proteasomal degradation. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 59343 Human
- Omim: 608261 Human
- SwissProt: Q9HC62 Human
- Unigene: 401388 Human
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Alternative names
- Axam2 antibody
- DKFZp762A2316 antibody
- KIAA1331 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
ab124724, at 1/500 dilution, staining SENP2 in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)](https://www.abcam.com/ps/products/124/ab124724/Images/ab124724-138545-anti-senp2-antibody-epr43562-western-blot.jpg)
Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)All lanes : Anti-SENP2 antibody [EPR4356(2)] (ab124724) at 1/10000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa -
![Anti-SENP2 antibody [EPR4356(2)] (ab124724)](https://www.abcam.com/ps/products/124/ab124724/Images/ab124724-3-benefits-of-recombinant-antibodies.png)
Anti-SENP2 antibody [EPR4356(2)] (ab124724)
Protocols
Datasheets and documents
References (1)
ab124724 has been referenced in 1 publication.
- Madu IG & Chen Y Assays for investigating deSUMOylation enzymes. Curr Protoc Mol Biol Chapter 10:Unit10.30 (2012). PubMed: 22870856
