Recombinant Anti-SENP2 antibody [EPR4356(2)] (ab124724)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SENP2 antibody [EPR4356(2)]
See all SENP2 primary antibodies -
Description
Rabbit monoclonal [EPR4356(2)] to SENP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SENP2 aa 350-450. The exact sequence is proprietary.
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Positive control
- MDA-MB-435, NIH:OVCAR-3, and 293T cell lysates; Human colon tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4356(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/250 - 1/500. |
Target
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Function
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. -
Sequence similarities
Belongs to the peptidase C48 family. -
Domain
The N-terminus is necessary and sufficient for nuclear envelope targeting. -
Post-translational
modificationsPolyubiquitinated; which leads to proteasomal degradation. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 59343 Human
- Omim: 608261 Human
- SwissProt: Q9HC62 Human
- Unigene: 401388 Human
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Alternative names
- Axam2 antibody
- DKFZp762A2316 antibody
- KIAA1331 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)](https://www.abcam.com/ps/products/124/ab124724/Images/ab124724-138544-anti-senp2-antibody-epr43562-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)ab124724, at 1/500 dilution, staining SENP2 in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)](https://www.abcam.com/ps/products/124/ab124724/Images/ab124724-138545-anti-senp2-antibody-epr43562-western-blot.jpg)
Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)All lanes : Anti-SENP2 antibody [EPR4356(2)] (ab124724) at 1/10000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Madu IG & Chen Y Assays for investigating deSUMOylation enzymes. Curr Protoc Mol Biol Chapter 10:Unit10.30 (2012). Read more (PubMed: 22870856) »
