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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Sumo
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RecombinantRabMAb

Recombinant Anti-SENP2 antibody [EPR4356(2)] (ab124724)

  • Datasheet
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
  • Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
  • Anti-SENP2 antibody [EPR4356(2)] (ab124724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4356(2)] to SENP2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Recombinant Human SENP2 protein (ab199592)

View more associated products

Overview

  • Product name

    Anti-SENP2 antibody [EPR4356(2)]
    See all SENP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4356(2)] to SENP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SENP2 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • MDA-MB-435, NIH:OVCAR-3, and 293T cell lysates; Human colon tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4356(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Associated products

  • Alternative Versions

    • Anti-SENP2 antibody [EPR4356(2)] - BSA and Azide free (ab247982)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SENP2 protein (ab199592)

Applications

Our Abpromise guarantee covers the use of ab124724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
    • Sequence similarities

      Belongs to the peptidase C48 family.
    • Domain

      The N-terminus is necessary and sufficient for nuclear envelope targeting.
    • Post-translational
      modifications

      Polyubiquitinated; which leads to proteasomal degradation.
    • Cellular localization

      Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
    • Target information above from: UniProt accession Q9HC62 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 59343 Human
      • Omim: 608261 Human
      • SwissProt: Q9HC62 Human
      • Unigene: 401388 Human
      • Alternative names

        • Axam2 antibody
        • DKFZp762A2316 antibody
        • KIAA1331 antibody
        • Senp2 antibody
        • SENP2_HUMAN antibody
        • Sentrin specific protease 2 antibody
        • Sentrin-specific protease 2 antibody
        • Sentrin/SUMO specific protease SENP2 antibody
        • Sentrin/SUMO-specific protease SENP2 antibody
        • SMT3 specific isopeptidase 2 antibody
        • SMT3-specific isopeptidase 2 antibody
        • Smt3ip2 antibody
        • SUMO1/sentrin/SMT3 specific peptidase 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [EPR4356(2)] (ab124724)

        ab124724, at 1/500 dilution, staining SENP2 in paraffin embedded Human colon tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
        Western blot - Anti-SENP2 antibody [EPR4356(2)] (ab124724)
        All lanes : Anti-SENP2 antibody [EPR4356(2)] (ab124724) at 1/10000 dilution

        Lane 1 : MDA-MB-435 cell lysate
        Lane 2 : NIH:OVCAR-3 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 68 kDa

      • Anti-SENP2 antibody [EPR4356(2)] (ab124724)
        Anti-SENP2 antibody [EPR4356(2)] (ab124724)

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab124724? Please let us know so that we can cite the reference in this datasheet.

      ab124724 has been referenced in 1 publication.

      • Madu IG & Chen Y Assays for investigating deSUMOylation enzymes. Curr Protoc Mol Biol Chapter 10:Unit10.30 (2012). PubMed: 22870856

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunocytochemistry/ Immunofluorescence abreview for Anti-SENP2 antibody [EPR4356(2)]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton 5 minutes
      Specification
      HeLa
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 22 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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