Overview

  • Product name

    Anti-SENP3 antibody [EP4360]
    See all SENP3 primary antibodies
  • Description

    Rabbit monoclonal [EP4360] to SENP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SENP3 aa 550-650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MCF-7, Jurkat and HeLa cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP4360
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
    • Sequence similarities

      Belongs to the peptidase C48 family.
    • Cellular localization

      Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress.
    • Information by UniProt
    • Database links

    • Alternative names

      • SENP3 antibody
      • SENP3_HUMAN antibody
      • Sentrin / SUMO specific protease 3 antibody
      • Sentrin specific protease 3 antibody
      • Sentrin-specific protease 3 antibody
      • Sentrin/SUMO-specific protease SENP3 antibody
      • SMT3IP1 antibody
      • SSP3 antibody
      • SUMO 1 specific protease 3 antibody
      • SUMO-1-specific protease 3 antibody
      • SUMO1 specific protease 3 antibody
      • SUMO1/sentrin/SMT3 specific peptidase 3 antibody
      • SUSP3 antibody
      see all

    Images

    • All lanes : Anti-SENP3 antibody [EP4360] (ab124790) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    References

    ab124790 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 17 2019

    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (MEF)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    20 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 07 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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