Key features and details
- Goat polyclonal to SENP6
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SENP6 antibody
See all SENP6 primary antibodies
DescriptionGoat polyclonal to SENP6
Specificityab77619 is expected to recognise both reported isoforms (NP_001093879.1; NP_056386.2).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
- HeLa cell nuclear lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77619 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProtease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison.
Tissue specificityHighly expressed in reproductive organs, such as testis, ovary and prostate.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the peptidase C48 family.
- Information by UniProt
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Anti-SENP6 antibody (ab77619) at 0.5 µg/ml + HeLa cell nuclear lysate in RIPA buffer at 35 µg
Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary antibody incubation: 1 hour
ab77619 has been referenced in 1 publication.
- Li Q et al. Ethanol Exposure Induces Microglia Activation and Neuroinflammation through TLR4 Activation and SENP6 Modulation in the Adolescent Rat Hippocampus. Neural Plast 2019:1648736 (2019). PubMed: 31781182