Overview

  • Product name

    Anti-SEP15 antibody [NCIR128A]
    See all SEP15 primary antibodies
  • Description

    Rabbit monoclonal [NCIR128A] to SEP15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse SEP15.

  • Positive control

    • Rat liver, fetal liver, human testis, mouse testis, rat testis and LnCaP lysates.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    NCIR128A
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

Target

Images

  • Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (purified) + Human testis lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (unpurified)

    Lane 1 : Rat liver lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : LnCaP liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Ekoue DN  et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). Read more (PubMed: 29314169) »
  • Touat-Hamici Z  et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . Read more (PubMed: 29660373) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 23 2018

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