Product nameAnti-SEP15 antibody [NCIR128A]
See all SEP15 primary antibodies
DescriptionRabbit monoclonal [NCIR128A] to SEP15
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse SEP15.
- Rat liver, fetal liver, human testis, mouse testis, rat testis and LnCaP lysates.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab124840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 18 kDa.|
FunctionMay be involved in redox reactions associated with the formation of disulfide bonds. May contribute to the quality control of protein folding in the endoplasmic reticulum.
Tissue specificityHigher levels in prostate and thyroid gland.
Sequence similaritiesBelongs to the selenoprotein M/SEP15 family.
modificationsThe N-terminus is blocked.
Cellular localizationEndoplasmic reticulum lumen. The association with UGGT1/UGCGL1 is essential for its retention in the endoplasmic reticulum.
- Information by UniProt
- 15 kDa selenoprotein antibody
- SELENOF antibody
- Selenoprotein F antibody
Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (purified) + Human testis lysates at 15 µg
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (unpurified)
Lane 1 : Rat liver lysate
Lane 2 : Fetal liver lysate
Lane 3 : LnCaP liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa
This product has been referenced in:
- Ekoue DN et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). Read more (PubMed: 29314169) »
- Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . Read more (PubMed: 29660373) »