Recombinant
RabMAb

Recombinant Anti-SEP15 antibody [NCIR128A] - BSA and Azide free (ab232465)

Overview

  • Product name

    Anti-SEP15 antibody [NCIR128A] - BSA and Azide free
    See all SEP15 primary antibodies
  • Description

    Rabbit monoclonal [NCIR128A] to SEP15 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse SEP15.
    Database link: Q9ERR7

  • Positive control

    • WB: Human testis lysate.
  • General notes

    Ab232465 is the carrier-free version of ab124840. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232465 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.

Target

Images

  • Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution + Human testis lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124840).

References

ab232465 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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